- ￥2630
- CUSABIO
- 武汉
- CSB-PA959449
- 2025年07月10日
- ELISA, WB, IHC, IF
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is di-methylated.
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
-20°C
- 克隆性：
多克隆
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12个月
- 抗原来源：
Human
- 目录编号：
CSB-PA959449
- 级别：
多克隆抗体
- 库存：
充足
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA, WB, IHC, IF
- 靶点：
HIST3H3
- 抗体英文名：
Histone H4R3me2s Antibody
- 规格：
100μl
| 产品名称 | Histone H4R3me2s Antibody |
| 货号 | CSB-PA959449 |
| 别名 | H4; H4/n; H4F2; H4FN; FO108; HIST2H4 |
| 抗体类型 | Polyclonal Antibody |
| 克隆类型 | Polyclonal |
| Uniprot No. | GeneID:8290SwissProt:Q16695 |
| 基因名 | HIST3H3 |
| 免疫原种属 | Human |
| 免疫原 | A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is di-methylated. |
| 宿主 | Rabbit |
| 反应种属 | Human, Mouse, Rat |
| 应用范围 | ELISA, WB, IHC, IF |
| 抗体亚型 | IgG |
| 标记方式 | Non-conjugated |
| 产品提供形式 | liquid |
| 纯化方式 | Antigen Affinity Purified |
| 研究领域 | Epigenetics and Nuclear Signaling |
| 保存缓冲液 | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 储存条件 | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
-Sepharose at 0.8 M NaCl, with H3/H4 subsequently eluted at 2 M NaCl. This procedure yields highly purified H2A/H2B and H3/H4. Finally, covalent chromatography on t hiop ropyl-S epharose (TPS) allows the separation of H3 from H4, by covalently binding H3
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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