- ￥2454
- CUSABIO
- 武汉
- CSB-PA938612
- 2025年07月13日
- ELISA, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of tyrosine 1110 (P-V-Y(p)-H-N) derived from Human EGFR.
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
-20°C
- 克隆性：
多克隆
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12个月
- 抗原来源：
Human
- 目录编号：
CSB-PA938612
- 级别：
多克隆抗体
- 库存：
充足
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA, WB
- 靶点：
EGFR
- 抗体英文名：
Phospho-EGFR (Tyr1110) Antibody
- 规格：
100μl
| 产品名称 | Phospho-EGFR (Tyr1110) Antibody |
| 货号 | CSB-PA938612 |
| 别名 | Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody; Cell growth inhibiting protein 40 antibody; Cell proliferation inducing protein 61 antibody; EGF R antibody; EGFR antibody; EGFR_HUMAN antibody; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody; Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody; Epidermal growth factor receptor antibody; erb-b2 receptor tyrosine kinase 1 antibody; ERBB antibody; ERBB1 antibody; Errp antibody; HER1 antibody; mENA antibody; NISBD2 antibody; Oncogen ERBB antibody; PIG61 antibody; Proto-oncogene c-ErbB-1 antibody; Receptor tyrosine protein kinase ErbB 1 antibody; Receptor tyrosine-protein kinase ErbB-1 antibody; SA7 antibody; Species antigen 7 antibody; Urogastrone antibody; v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody; wa2 antibody; Wa5 antibody |
| 抗体类型 | Polyclonal Antibody |
| 克隆类型 | Polyclonal |
| Uniprot No. | P00533 |
| 基因名 | EGFR |
| 免疫原种属 | Human |
| 免疫原 | Peptide sequence around phosphorylation site of tyrosine 1110 (P-V-Y(p)-H-N) derived from Human EGFR. |
| 宿主 | Rabbit |
| 反应种属 | Human, Mouse, Rat |
| 应用范围 | ELISA, WB |
| 抗体亚型 | IgG |
| 标记方式 | Non-conjugated |
| 产品提供形式 | liquid |
| 纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugateds. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 研究领域 | Signal Transduction |
| 保存缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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文献和实验【求助】干扰一个基因表达后，EGFR的表达及tyr1173磷酸化都降低，如何分析
zxlbaby 干扰一个基因表达后，EGFR的表达及tyr1173磷酸化都降低，如何分析？能说此基因具有调节EGFR的表达和磷酸化作用吗？还是磷酸化减少是因为表达减少？郁闷中。求助。 amygdala 用WB检测以后最好用条带扫描软件做一个量化统计分析，看看总EGFR和Tyr1173降低的比例是否一致，这样就可以知道是哪种情况了。 lilili1979 同意楼上的,可以考虑用磷酸化的/总蛋白
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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