- ￥2454
- CUSABIO
- 武汉
- CSB-PA261382
- 2025年07月11日
- ELISA, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of serine 71 (P-L-S(p)-Y-P) derived from Human Rac1.
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
-20°C
- 克隆性：
多克隆
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12个月
- 抗原来源：
Human
- 目录编号：
CSB-PA261382
- 级别：
多克隆抗体
- 库存：
充足
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA, WB
- 靶点：
RAC1
- 抗体英文名：
Phospho-RAC1 (Ser71) Antibody
- 规格：
100μl
| 产品名称 | Phospho-RAC1 (Ser71) Antibody |
| 货号 | CSB-PA261382 |
| 别名 | Cell migration inducing gene 5 protein antibody; Cell migration-inducing gene 5 protein antibody; MGC111543 antibody; MIG5 antibody; Migration inducing gene 5 antibody; Migration inducing protein 5 antibody; p21 Rac1 antibody; p21-Rac1 antibody; Rac 1 antibody; RAC1 antibody; RAC1_HUMAN antibody; Ras like protein TC25 antibody; Ras related C3 botulinum toxin substrate 1 (rho family; small GTP binding protein Rac1) antibody; Ras-like protein TC25 antibody; Ras-related C3 botulinum toxin substrate 1 antibody; Rho family small GTP binding protein Rac1 antibody; TC 25 antibody; TC25 antibody |
| 抗体类型 | Polyclonal Antibody |
| 克隆类型 | Polyclonal |
| Uniprot No. | P63000 |
| 基因名 | RAC1 |
| 免疫原种属 | Human |
| 免疫原 | Peptide sequence around phosphorylation site of serine 71 (P-L-S(p)-Y-P) derived from Human Rac1. |
| 宿主 | Rabbit |
| 反应种属 | Human, Mouse, Rat |
| 应用范围 | ELISA, WB |
| 抗体亚型 | IgG |
| 标记方式 | Non-conjugated |
| 产品提供形式 | liquid |
| 纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugateds. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
| 研究领域 | Signal Transduction |
| 保存缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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文献和实验antibody 进行Western blot. 有人使用这个试剂盒对贴壁细胞（人动脉内皮细胞，鼠动脉平滑肌细胞）、非贴壁细胞（鼠的淋巴细胞）、以及整个组织样本（猪的心）. 试剂盒对贴壁细胞和非贴壁细胞的说明非常清楚，但是对在组织中的使用却并不明确. 下面是在一篇文章中的实际应用 Pull-Down Assay for GTP-Rac1 HCE cells 在铺设了fibronectin和加或不加BSA 的100-mm dishes培养45分钟，在 0.5 mL of a solution中裂解
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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