Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围:
ELISA, WB
宿主:
Rabbit
适应物种:
Human
保质期:
6-12个月
抗原来源:
详询
目录编号:
orb763088
级别:
科研
库存:
99
供应商:
Biorbyt
标记物:
Unconjugated
克隆性:
POLYCLONAL
保存条件:
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
形态:
Lyophilized
亚型:
Rabbit IgG
免疫原:
E.coli-derived human TGFBR1 recombinant protein (Position: L34-K490).
Western blot analysis of TGF Beta Receptor I/TGFBR1 using anti-TGF Beta Receptor I/TGFBR1 antibody (orb763088). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor I/TGFBR1 antigen affinity purified polyclonal antibody (Catalog # orb763088) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor I/TGFBR1 at approximately 56 kDa. The expected band size for TGF Beta Receptor I/TGFBR1 is at 56 kDa.