Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围:
ELISA, FC, ICC, IF, WB
宿主:
Rabbit
适应物种:
Human
保质期:
6-12个月
抗原来源:
详询
目录编号:
orb745977
级别:
科研
库存:
99
供应商:
Biorbyt
标记物:
Unconjugated
克隆性:
POLYCLONAL
保存条件:
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
形态:
Lyophilized
亚型:
Rabbit IgG
免疫原:
E.coli-derived human DAI/ZBP1 recombinant protein (Position: A2-E421).
Flow Cytometry analysis of U87 cells using anti-DAI/ZBP1 antibody (orb745977). Overlay histogram showing U87 cells stained with orb745977 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAI/ZBP1 Antibody (orb745977, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IF analysis of DAI/ZBP1 using anti-DAI/ZBP1 antibody (orb745977). DAI/ZBP1 was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-DAI/ZBP1 Antibody (orb745977) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of DAI/ZBP1 using anti-DAI/ZBP1 antibody (orb745977). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HeLa whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAI/ZBP1 antigen affinity purified polyclonal antibody (Catalog # orb745977) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for DAI/ZBP1 at approximately 63KD. The expected band size for DAI/ZBP1 is at 63KD.