Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围:
ELISA, FC, IHC, WB
宿主:
Rabbit
适应物种:
Human
保质期:
6-12个月
抗原来源:
详询
目录编号:
orb738391
级别:
科研
库存:
99
供应商:
Biorbyt
标记物:
Unconjugated
克隆性:
POLYCLONAL
保存条件:
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
形态:
Lyophilized
亚型:
Rabbit IgG
免疫原:
E.coli-derived human CARD4/NOD1 recombinant protein (Position: N26-F953).
IHC analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody (orb738391). CARD4/NOD1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CARD4/NOD1 Antibody (orb738391) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.
Western blot analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody (orb738391). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4/NOD1 antigen affinity purified polyclonal antibody (Catalog # orb738391) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for CARD4/NOD1 at approximately 107KD. The expected band size for CARD4/NOD1 is at 107KD.
Flow Cytometry analysis of CACO-2 cells using anti-CARD4/NOD1 antibody (orb738391). Overlay histogram showing CACO-2 cells stained with orb738391 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CARD4/NOD1 Antibody (orb738391, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.