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文献和实验Baculovirus Expression Vectors
This chapter aims to provide the reader with the experimental protocols required to produce a baculovirus containing the gene of interest, including transfection of Spodoptera frugiperda cells and the screening of progeny virus by plaque assay
Generation of baculovirus expression vectors
The baculovirus expression system has become an important tool for the expression of heterologous genes because it has several positives attributes. First, high quantities of protein are produced because the target genes are driven by strong
Use of Baculovirus Expression Vectors
The baculovirus expression vector system is a helper-independent system that has found extensive use in the past decade for the expression of heterologous genes. Its popularity stems from a combination of high levels of expression
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