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文献和实验isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
Figure 17.15.4 Defining a useful dilution of human plasma for measuring 3‐nitrotyrosine. ELISA microarray results for the dilution of human plasma. Four samples were diluted with 0.1% BSA/PBS at 2‐, 5‐, 10
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