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文献和实验Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases
Catabolism of extracellular matrix (ECM) components has been ascribed to a family of Zn2� metalloenzymes. These matrix metalloproteinases (MMPs; also termed matrixins) are believed to be important in connective tissue remodeling
This chapter describes a number of aspects important for calibration of matrix-assisted laser desorption/ionization time-of-flight spectra prior to peptide mass fingerprinting searches. Both multipoint internal calibration
properties such as superior light absorption. Here we report methods for improving matrix-assisted laser desorption/ionization desorption of crude tryptic protein digests by using CdSe/ZnS QDs. The addition of QDs to the matrix improves the signal-to-noise
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