10–50 μg HeLa 细胞裂解液中三种管家蛋白的免染色总蛋白信号与化学发光信号的线性比较。 (A)免染总蛋白;(B、C、D)β-actin等三种管家蛋白;(E)免染总蛋白、三种管家蛋白的线性比较。尽管actin与tubulin信号呈线性,但光密度远低于实际加载的预测“定量响应”,而免染总蛋白信号与预期结果高度一致。
参考文献:Taylor SC Posch A (2014). The design of a quantitative western blot experiment. Biomed Res Int. 36, 1590
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