产品名称:Recombinant Human ECM1 宿主: E.Coli 标签: N-terminal His-IF2DI Tag 规格: 50μg, 200μg, 1mg 种属: Human 表达区间: 24-210 分子量: 41.3 kDa 纯度: Greater than 90% by SDS-PAGE gel analyses 应用: Western Blot, ELISA 性状: Lyophilized from a 0.2μm filtered solution in PBS with 5% trehalose, pH7.4 溶解方法: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex 存储: -20°C for 12 months as lyophilized; 2-8°C for 1 month under sterile conditions after reconstitution 别名: ECM 1, Ecm1, ECM1_HUMAN, Extracellular matrix protein 1, Secretory component p85, URBWD
Recombinant Human KI67宿主: E.Coli标签: N-terminal His Tag种属: Human表达区间: 762-1016分子量: 30 KDa纯度: Greater than 95% by SDS-PAGE gel analyses应用: Western Blot, ELISA性状: Lyophilized from a 0.2μm filtered solution in PBS with 5% trehalose, pH7.4溶解方法: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex存储: -20°C for 12 months as lyophilized; 2-8°C for 1 month under sterile conditions after reconstitution别名: Antigen KI 67, Ki 67, KI67, KIA, MKI67 Recombinant Human APT-2宿主: E.Coli标签: N-terminal His Tag种属: Human表达区间: 1-231分子量: 25.3 kDa纯度: Greater than 95% by SDS-PAGE gel analyses应用: Western Blot, ELISA性状: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4溶解方法: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex存储: -20°C for 12 months as lyophilized; 2-8°C for 1 month under sterile conditions after reconstitution别名: Acyl protein thioesterase, Acyl protein thioesterase 2, Acyl-protein thioesterase 2, APT 2, APT-2, APT2, DJ886K2.4, EC 3.1.2.-, LPL I, LPL II, LPL-II, LYPA2_HUMAN, LYPLA 2, Lypla2, Lysophospholipase II, LysoPLA II, OTTHUMP00000002999, OTTHUMP00000044831 操作步骤: Ⅰ 实体组织蛋白的提取 1. 在每1mL 冷Lysis Buffer加入10 μL磷酸酶抑制剂, 1 μL蛋白酶抑制剂和 5μL 100mM PMSF,混匀。冰上保存数分钟待用。 2. 每100mg固体组织置于培养皿中,手术剪剪碎成3mm×3mm左右的小块,加入0.5~1 mL冷 Lysis Buffer,玻璃匀浆器上下手动匀浆15次,注意低温操作; 3. 取组织匀浆液转移到1.5mL预冷的离心管,离心10000转/分,4℃离心5 min; 4. 取上清转移至新的预冷的离心管中,即为全蛋白提取物,蛋白定量(Bradford法、BCA法); 5. 分装保存于-70℃,避免反复冻融。 Ⅱ 培养细胞蛋白提取 1. 贴壁培养的细胞,吸去培养基后,加入10mL/150mm培养板的冷PBS洗两次,每次振摇数次以尽量去除培养液; 2. 悬浮培养的细胞或用细胞刮子刮下的贴壁细胞,将细胞及培养液移至离心管中, 1000转/分离心10 min,再用10mL/150mm培养板的冷PBS,1000转/分离心5 min洗两次; 3. 在每 1mL冷 Lysis Buffer加入10μL磷酸酶抑制剂, 1μL蛋白酶抑制剂和5μL 100mM PMSF,混匀。冰上保存数分钟待用。 4. 细胞洗涤后,转至新的预冷的离心管中,加入上述配制好的冷Lysis Buffer,加入量如下表所示:
细胞数量
Lysis Buffer加入量
107个
0.5 mL~1 mL
5×106个
0.2 mL~0.5 mL
5.置于4℃摇床平台上,温和振荡15 min; 6. 14,000rpm,4℃离心15min,取上清为全蛋白提取物, 蛋白定量(Bradford法、BCA法); 7. 分装保存于-70℃,避免反复冻融。 肌原调节蛋白抗体Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep英文名称: MyoD1/Myf3抗体来源: Rabbit中文名称: 肌原调节蛋白抗体浓度: 1mg/ml交叉反应: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep标记: Unconjugated性状: Liquid抗体类型: Polyclonal免疫原: KLH conjugated synthetic peptide derived from mouse MyoD1蛋白细胞定位: 细胞核纯化方法: affinity purified by Protein A亚型: IgG 自噬微管相关蛋白轻链3抗体Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, Goat英文名称: LC3抗体来源: Rabbit中文名称: 自噬微管相关蛋白轻链3抗体浓度: 1mg/ml交叉反应: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, Goat标记: Unconjugated性状: Liquid抗体类型: Polyclonal免疫原: KLH conjugated synthetic peptide derived from human LC3蛋白细胞定位: 细胞浆纯化方法: affinity purified by Protein A亚型: IgG Recombinant Human ECM1蛋白Human Bone Morphogenetic Protein 7(BMP7)ELISA Kit人骨成型蛋白7(BMP7)ELISA试剂盒Human/人Elisa试剂盒48T Human Bone Morphogenetic Protein 4(BMP4)ELISA Kit人骨成型蛋白4(BMP4)ELISA试剂盒Human/人Elisa试剂盒48T Human Bone Morphogenetic Protein 3(BMP3)ELISA Kit人骨成型蛋白3(BMP3)ELISA试剂盒Human/人 产品特点: 1.使用方便,从细胞,组织中提取蛋白不需要经过研磨、反复冻融、超声破碎等前处理。 2.将蛋白提取的时间缩短至30分钟-1小时。 3.含蛋白稳定剂,提取的蛋白稳定。 4.紫外检测蛋白浓度时,背景干扰低。 5.蛋白酶抑制剂抑制了蛋白的降解,蛋白酶抑制剂配方优化。蛋白酶抑制剂混合物包含6种独立的蛋白酶抑制剂;每一种抑制剂可特异性抑制某一种或几种蛋白酶活性。该混合物优化的组成使其可以抑制几乎所有重要的蛋白酶活性,包括丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶、丙氨酰-氨基肽酶等。