产品名称:C1orf51抗体 产品分类 抗体 / 一抗 英文名称 :C1orf51 中文名称:1号染色体开放阅读框51抗体 英文别名 :C1orf51; CA051_HUMAN; FLJ25889; Uncharacterized protein C1orf51. 交叉反应 :Human, Mouse, Rat, Dog, Pig, Cow, Horse 标记 :Unconjugated 抗体来源:Rabbit 抗体类型:Polyclonal 免疫原:KLH conjugated synthetic peptide derived from human C1orf51 蛋白细胞定位:细胞核 纯化方法:affinity purified by Protein A 亚型:IgG 性状:Liquid 浓度:1mg/ml 储存液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 背景资料 Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human ome. There are about 3,000 es on chromosome 1, and considering the great number of es there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA e which encodes lamin A. When defective, the LMNA e product can build up in the nucleus and cause characteristic nuclear blebs. The mechanism of rapidly enhanced aging is unclear and is a topic of continuing exploration. The MUTYH e is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are also associated with chromosome 1. A breakpoint has been identified in 1q which disrupts the DISC1 e and is linked to schizophrenia. Aberrations in chromosome 1 are found in a variety of cancers including head and neck cancer, malignant melanoma and multiple myeloma. The C1orf51 e product has been provisionally designated C1orf51 pending further characterization. There are two isoforms of C1orf51 that are produced as a result of alternative splicing events. 抗体的制备过程: 1. 免疫原的制备 普通的大分子蛋白,通过分子克隆构建载体并在大肠杆菌中进行诱导表达获得重组蛋白,纯化鉴定后可直接作为免疫原。 小分子蛋白或化合物等分子量小,需要偶联载体对该分子进行改造才能使其成为具有免疫原性的抗原,常见偶联载体如BSA、OVA、HAS等。 2. 免疫动物 常用于制备抗血清的动物有豚鼠、家兔、鸡、大小鼠等,大量生产时需要用到狗、绵羊、山羊等。 3. 免疫血清的收集 一般家兔、绵羊、山羊可采用静动脉采血,家兔、豚鼠、大鼠、鸡可采用心脏采血,家兔、山羊、绵羊可采用静脉采血。 4. 免疫血清的纯化与鉴定 得到的抗血清需要进一步的纯化,利用偶联了抗原的亲和柱进行层析,具有高效,特异性强,纯度高的特定。接着要鉴定纯化蛋白的含量、相对分子的质量、纯度以及特异性。 5. 免疫血清的保存 建议将抗体分装后进行保存。抗体一般比较稳定,在-80℃ ~-20 ℃可以保存约5年而不会影响效价,而真空干燥保存时间可以更久。保存前需经除菌并添加防腐剂。产品仅用于科研 下列是公司正在促销出售的产品: 小鼠少突胶质前体细胞(MOPC)(5×105) Hep-2, 人喉癌细胞系 Human葫芦素E(标准品)质量规格:HPLC≥95%,标准品Cucurbitacin E 中国仓鼠卵巢细胞(亚系克隆);CHO-HCV-NS3钩吻素子(标准品)质量规格:HPLC≥98%,标准品Koumine 人肝内胆管上皮细胞 (HIBEC)( 5×105 )钩吻素甲(标准品)质量规格:HPLC≥98%,标准品Gelsemine CL-0059CFPAC-1(人胰腺癌细胞)5×106cells/瓶×2原B1(标准品)质量规格: CD47 Others Rat 大鼠 CD47 人细胞裂解液 (阳性对照) 十五烷酸1克 人角膜上皮细胞完全培养基 100mL肌激酶 Myokinase from chicken muscle 9013-2-9 1KU 通用试剂 HPT-8细胞,小鼠饲养层上皮细胞 293(转染腺病毒E1A基因的人肾上皮细胞) 长白山猪胚胎皮肤成纤维样细胞;201184衣康腊酐 Itcconic cnhydridq 170-3-8 EFNB2 Protein Human 重组人 Ephrin-B2 / EFNB2 蛋白 ( C1orf51抗体NCI-H209细胞,人小细胞肺癌 人癌抗雌激素耐药株,LCC9细胞 上皮细胞生长添加物MEpiCGS(1-癸基)基溴化,英文名或英文缩写:Decyltrimetxylammonium bromide,级别:超,99%,规格:5克 水牛皮肤成纤维样细胞;WB-S5-beta-蒎1(2-8℃) (1S)-(1)-bqtc-Pinqnq 1817-67-3 IL1RL1 Others Human 人 IL1RL1 / DER4 人细胞裂解液 (阳性对照) 流醋 LqcD(II) SULFcTq 7446-14- 恒河猴肺细胞;RM-L1PROTEASEINHIBCKTL,GENERALW/EDTA通用蛋白酶抑制剂混合物,含EDTA蛋白组学级1MLFrozen 8. 骨骼肌细胞系统2480-23-1N-甲基-L-缬酸H-MEVAL-OH HCL