Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
浓度:
1mg/ml
规格:
100ul
产品属性:
产品名称
信号通路Wnt2B重组兔单克隆抗体
浓度
1mg/ml
型号
EY-01K6116
产品名称:信号通路Wnt2B重组兔单克隆抗体 英文名称:Wnt2b 英文别名:wingless-type MMTV integration site family, member 2B; XWNT2; WNT13; WNT2B_HUMAN. 交叉反应: Human, Mouse, Rat 标记: Unconjugated 抗体来源: Rabbit 抗体类型: Monoclonal 克隆号: 2G5 免疫原: Recombinant human Wnt2b 蛋白细胞定位: 细胞外基质,分泌型蛋白 纯化方法: affinity purified by Protein A 亚型: IgG 性状: Liquid 浓度: 1mg/ml 储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件: Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. 背景资料: WNT2B is a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis. This gene produces two alternative transcript variants.This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis. This gene produces two alternative transcript variants. 抗体分子标记技术: (一)原理 当应用化学氧化法时(NaI)遇强氧化剂,离子被氧化为分子,所生成的自由分子可与某些基团进行卤化反应。蛋白质分子可进行卤化反应的基团主要为酪氨酸残基,某些组氨酸残基也可能进行化。在应用胺T( Chloramine T)法的实验中,所用的氧化剂(1,3,4,6- tetrachloro-3a,6adipheny l-glucoluril)强挥发性的有机溶剂中。该溶剂加入试管后,先让其挥发(即让氧化剂将试管包被),然后把Na125I和蛋白质液加入包被好的试管中,反应完成即将混合液移入他管终止反应。 (二)准备 1.试剂 经亲和层析纯化的多克隆抗体或单克隆抗体;0.5mol/L磷酸钠缓冲液,pH7.5;无载体的Na125I3.7GBq/ml(100mCi/ml)的NaOH液;100g/L三醋酸;70%乙醇;新鲜制备的含2mg/m1络胺T的0.5mol/L磷酸钠缓冲液(pH7.5);胺T反应终止缓冲液; 2.仪器 凝胶过滤柱;¥-记数器;玻璃纤维滤。 (三)操作要点 1.用胺T法进行化,也可在大约pH7.0的缓冲液中进行; 2.如果氧化反应损伤蛋白质,可将抗体与异硫酸荧光素(FITC)偶联)胺T量减少到0.02mg/m,并将偏重亚硫酸液的浓度降到0.024mg/ml; 3. Iodogen-包被的试管可在干燥器中,于室温下保存多年; 4.1251标记的抗体,在制备后六周内均可使用(1251的半衰期为59.6天); 5.要注意保证所用的Na1251是新鲜的,陈旧制剂的比活性低; 6.酪氨酸的碘化偶尔会对抗原的抗体结合位点有干扰,因此降低其结合能力,但这种情况很少见。 抗体制备过程: 1.材料与试剂 a.提取的动物 Ig b.弗氏完全佐剂和弗氏不完全佐剂 c.青霉素和链霉素 d.实验动物 兔 e.其它材料及试剂 2、选择实验活体。 3、进行动物免疫实验。 4、试取血样进行测试,查看免疫效果。 5、如果免疫成功,杀死实验活体,采集全部血清。 6、纯化出抗体。 7、鉴定抗体。胎牛血清(无菌采制) 凋亡相关激酶检测试剂盒 DAPK ELISA KitHuman DAPK ELISA Kit 凋亡相关半胱氨酸肽酶4检测试剂盒 Casp4 ELISA Kithuman caspase 4-apoptosis-related cysteine peptidase,Casp4 ELISA Kit 凋亡酶活化1检测试剂盒 APAF-1 ELISA KitHuman apoptotic protease activating factor-1,Apaf-1 ELISA Kit 淀粉状β检测试剂盒 FE65 ELISA KitHuman FE65 ELISA kit 电子转运黄素-泛醌氧化还原酶/电子转运黄素脱氢酶检测试剂盒 ETFDH ELISA KitHuman ETFDH ELISA kit 电压门控钾通道抗体检测试剂盒 VGKC-ab ELISA KitHuman voltage-gated potassium channel antibody,VGKC-ab ELISA Kit 电压门控钾通道检测试剂盒 VGKC ELISA KitHuman voltage-gated potassium channel,VGKC ELISA Kit 0号染色体缺失并与张力同源的磷酸酶检测试剂盒 PTEN/MMAC1 ELISA KitHuman PTEN ELISA Kit 抵抗素样分子β检测试剂盒 RELM-β ELISA KitHuman RELM-β ELISA Kit 抵抗素检测试剂盒 Resistin ELISA Kithuman Resistin ELISA Kit 信号通路Wnt2B重组兔单克隆抗体Phospho-CD18 (Thr758)/RBITC罗丹明(RBITC)标记的磷酸化整合素β2/Integrin β2抗体 LYPD4/RBITC罗丹明(RBITC)标记的LYPD4抗体 GAS41/RBITC罗丹明(RBITC)标记的NuBI结合1抗体 Phospho-IRAK1 (Thr387)/RBITC罗丹明(RBITC)标记的磷酸化白介素-1受体相关激酶1抗体 HEATR2/RBITC罗丹明(RBITC)标记的HEATR2抗体 PLOD3/RBITC罗丹明(RBITC)标记的赖氨酸羟化酶3抗体 FKBP38/RBITC罗丹明(RBITC)标记的FK506结合38抗体 phospho-MDC1 (Thr4)/RBITC罗丹明(RBITC)标记的磷酸化DNA损伤关卡1抗体