运输和保存方法 冰袋运输。粉末直接保存于-20℃,有效期2年。建议分装后-20℃干燥保存,避免反复冻融。 注意事项 1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。 2. 粉末溶解前请先短暂离心,以保证产品全在管底。 3. 请勿吸入、吞咽或者直接接触皮肤和眼睛。 4. 本产品仅用于科研用途,禁止用于人身上。 使用浓度 【具体使用浓度请参考相关文献,并根据自身实验条件(如实验目的,细胞种类,培养特性等)进行摸索和优化。】 使用方法(数据来自于公开发表的文献,仅供参考) (一)细胞实验(体外实验) 细胞按照2~3×103密度接种于96孔板中孵育24 h后,培养基配置的MK-2206(0,0.3 μM,1 μM,3 μM)处理96孔中的细胞,72 h或96 h后测定细胞增殖情况,MK-2206增强拉帕替尼诱导的细胞凋亡。[1] (二)动物实验(体内实验) MK-2206 (120 mg/kg)和厄洛替尼(50 mg/kg)口服处理NCI-H292肿瘤移植小鼠模型,14 h后检测其药效学和抗肿瘤作用,Western blot分析发现,MK-2206中度(53.1±6.2%)抑制phospho-Akt通路,轻度(27.7±5.9%)抑制phospho-Erk通路,MK-2206和厄洛替尼联合使用增强了对这两种通路的抑制作用。[1] 参考文献 [1]. Hirai H, et al. MK-2206, an allosteric Akt inhibitor, enhances antitumor efficacy by standard chemotherapeutic agents or molecular targeted drugs in vitro and in vivo. Mol Cancer Ther. 2010 Jul;9(7):1956-67. [2]. Cheng Y, et al. eEF-2 kinase dictates cross-talk between autophagy and apoptosis induced by Akt Inhibition, thereby modulating cytotoxicity of novel Akt inhibitor MK-2206. Cancer Res. 2011 Apr 1;71(7):2654-63. [3]. Cui H, et al. The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model. Mol Med Rep. 2020 Nov;22(5):3723-3734. HB230119
[1]. Hirai H, et al. MK-2206, an allosteric Akt inhibitor, enhances antitumor efficacy by standard chemotherapeutic agents or molecular targeted drugs in vitro and in vivo. Mol Cancer Ther. 2010 Jul;9(7):1956-67. [2]. Cheng Y, et al. eEF-2 kinase dictates cross-talk between autophagy and apoptosis induced by Akt Inhibition, thereby modulating cytotoxicity of novel Akt inhibitor MK-2206. Cancer Res. 2011 Apr 1;71(7):2654-63. [3]. Cui H, et al. The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model. Mol Med Rep. 2020 Nov;22(5):3723-3734.