- 询价
- Biotechrabbit
- BR0502002
- 2026年01月27日
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4X CAPITAL™ 1-Step qRT-PCR Probe Master Mix
Features
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Best in-class performance for both single and multiplex detection
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Convenient master mix for detection of low-copy pathogen targets
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High specificity and sensitivity across a wide range of sample sources
Applications
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One step qRT-PCR from mRNA, total RNA and viral RNA targets
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For use with standard and fast qPCR platforms
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Single and multiplex qRT-PCR reactions
订购信息：
产品名称：4X CAPITAL™ 1-Step qRT-PCR Probe Master Mix
产品货号：BR0502002
产品规格：1000 reactions of 20 µl
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文献和实验at 4°C--> remove the supernatent--> dry the pellet in the speed vac centrifuge for 5-10 min--> solve the pellet in 5 µl formamide, incubate the probe for 10 min at 37°C, add 10 µl of master mix 2. Denaturation of the genomic DNA and prehybridization
ºC, x3. 6. Blocking step: Wipe off back of slide. Add 100 µL block solution (4x SSC, 5% milk, 0.1% Tween-20) to the slide in 2 spots. (To store blocking reagent add 0.025% NaAzide and refrigerate at 4ºC) Cover with a large glass slip
in 2X SSC for 5 minutes each at 42°C; If a biotin-labelled probe (e.g. chromosome paint) is used, a detection step has to be included; Block in 4X SSC/0.1% Tween/5% BSA (Gibco) for 15 minutes at RT; Incubate in fluorescently
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