AcceGen人B淋巴白血病细胞NALM-6细胞
产品简介:
产品英文名称:NALM-6;
货号:ABC-TC252S;
物种:人类;
产品类型:人类白血病细胞系;
描述:来源于B细胞白血病的人细胞系。使用寿命:无限。
生长特征:淋巴细胞样;
推荐培养基和补充剂:RPMI1640+10%FBS;
生物安全等级:一级;
运输方式:干冰运输;
储存方式:液氮储存;
应用:仅供研究使用。
The NALM-6 cell line originated from blood cultures collected from a 19-year-old male patient who had been diagnosed with non-T, non-B acute lymphoblastic leukemia (ALL) during a relapse. These cultures yielded 8 “leukemia” and 2 “normal-B” cell lines. Interestingly, at the time of culture, the leukemic blasts displayed a unique membrane phenotype, characterized by the presence of SmIg (lambda and mu chain immunoglobulins), Ia-like B cell associated antigen (Ia), and the common form of ALL specific antigen (cALL). This phenotype differed from the typical non-T, non-B ALL phenotype seen at the disease’s onset. Among the 8 established leukemia cell lines (designated as NALM 6-13), variations in membrane phenotype expression were evident, despite the shared cytogenetic markers (5q- and 12p+) and monoclonal SmIg (lambda, mu). These observations suggest the potential for limited intra-clonal differentiation within the leukemia clone.
Why choose NALM-6 from AcceGen?
The NALM-6 cell line from AcceGen is characterized by its high viability and exceptional quality, maintained through stringent quality control measures. These cells are consistently monitored for sterility, ensuring the absence of bacteria and fungi, and confirmed to be negative for mycoplasma using PCR-based assays. Furthermore, they have been rigorously tested and found to be free of human pathogens such as EBV, HBV, HCV, HHV-8, HIV-1, HIV-2, HTLV-1/2, MLV, and SMRV, guaranteeing their suitability for diverse research applications.
FOR RESEARCH USE ONLY
NALM-6 cell line finds valuable applications in the study of human acute lymphoblastic leukemia, providing essential insights into the disease and serving as a foundational tool for potential treatment strategies. Researchers use NALM-6 to explore various aspects of leukemia, including the detection and potential accumulation of oxidative clustered DNA lesions in leukemia cells when exposed to radiation doses akin to those employed in radiotherapy. These investigations not only deepen our understanding of leukemia but also contribute to the development of innovative therapeutic approaches for this challenging condition.
细胞培养:
1、培养前准备
(1)培养器具:推荐使用25 cm2培养瓶或60 mm培养皿(或更小)。
(2)培养基:准备适合培养AcceGen人B淋巴白血病细胞NALM-6细胞的培养基,建议用量5 mL(或更少)。
2、细胞解冻
将包装中的冻存管取出后立即放在不高于37℃的水中,并在2分钟内摇晃解冻。
3、细胞复苏
(1)取10ml培养基置于离心管中,用70%酒精或阳离子洗涤剂渗透纱布消毒冻存管,用消毒纱布包裹管身,拧开管盖后,将细胞悬浮液转移到离心管中;
(2)1000 rpm离心3分钟,弃掉上清液;
(3)将细胞重新悬浮在5ml上述培养基中(不清洗细胞)。在25 cm2的培养瓶或60 mm的培养皿中进行培养。
4、细胞传代
在传代之前,确保细胞增殖,因为过度稀释可能导致细胞死亡,尤其是血细胞和淋巴细胞。
5、细胞冻存
待AcceGen人B淋巴白血病细胞NALM-6细胞生长状态良好时,尽早进行细胞冻存。
产品信息:
货号 |
产品名称 |
规格 |
ABC-TC252S |
NALM-6 |
1 vial |