- ￥1080 - 3200
- 上海一研
- EY-01K3289
- 国产
- 2025年07月13日
- 说明书
- 说明书
- 说明书
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- 详细信息
- 技术资料
- 供应商：
一研
- 库存：
44
- 靶点：
说明书
- 级别：
说明书
- 目录编号：
说明书
- 克隆性：
单克隆
- 抗原来源：
Rabbit
- 保质期：
说明书
- 抗体英文名：
IL-4
- 抗体名：
白介素4重组兔单克隆抗体
- 标记物：
Unconjugated
- 宿主：
说明书
- 适应物种：
说明书
- 免疫原：
Recombinant human IL-4 protein
- 亚型：
LgG
- 形态：
液体
- 应用范围：
说明书
- 保存条件：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 浓度：
1mg/ml
- 规格：
100ul
产品属性：
产品名称：白介素4重组兔单克隆抗体
英文名称：IL-4
英文别名:Interleukin-4; IL4; IL 4; B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; BCGF 1; BCGF1; Binetrakin; BSF 1; BSF1; HCGF; Hodgkin’s Cell Growth Factor; IA Inducing Factor; IGG1 induction factor; Il4e12; Interleukin 4; Interleukin 4, isoform 1; Interleukin4; Lymphocyte stimulatory factor 1; Macrophage fusion factor; Mast cell growth factor 2; MCGF2; MFF; MGC79402; Pitrakinra; T cell growth factor 2; TCGF2; IL4_HUMAN; B-cell stimulatory factor 1; BSF-1.
交叉反应: Human, Mouse
标记: Unconjugated
抗体来源: Rabbit
抗体类型: Monoclonal
免疫原: Recombinant human IL-4 protein
纯化方法: affinity purified by Protein A
亚型: IgG
性状: Liquid
浓度: 1mg/ml
储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件: Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
背景资料: The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020]
抗体制备过程：
1.材料与试剂
a．提取的动物 Ig
b．弗氏完全佐剂和弗氏不完全佐剂
c．青霉素和链霉素
d．实验动物 兔
e．其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试，查看免疫效果。
5、如果免疫成功，杀死实验活体，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清（无菌采制）
抗体分子标记技术：
（一）原理
当应用化学氧化法时(NaI)遇强氧化剂,离子被氧化为分子,所生成的自由分子可与某些基团进行卤化反应。蛋白质分子可进行卤化反应的基团主要为酪氨酸残基,某些组氨酸残基也可能进行化。在应用胺T( Chloramine T)法的实验中,所用的氧化剂(1,3,4,6- tetrachloro-3a,6adipheny l-glucoluril)强挥发性的有机溶剂中。该溶剂加入试管后,先让其挥发(即让氧化剂将试管包被),然后把Na125I和蛋白质液加入包被好的试管中,反应完成即将混合液移入他管终止反应。
（二）准备
1.试剂
经亲和层析纯化的多克隆抗体或单克隆抗体；0.5mol/L磷酸钠缓冲液,pH7.5；无载体的Na125I3.7GBq/ml(100mCi/ml)的NaOH液；100g/L三醋酸；70%乙醇；新鲜制备的含2mg/m1络胺T的0.5mol/L磷酸钠缓冲液(pH7.5）；胺T反应终止缓冲液；
2.仪器
凝胶过滤柱；￥-记数器；玻璃纤维滤。
（三）操作要点
1.用胺T法进行化,也可在大约pH7.0的缓冲液中进行；
2.如果氧化反应损伤蛋白质，可将抗体与异硫酸荧光素(FITC)偶联)胺T量减少到0.02mg/m，并将偏重亚硫酸液的浓度降到0.024mg/ml；
3. Iodogen-包被的试管可在干燥器中,于室温下保存多年；
4.1251标记的抗体,在制备后六周内均可使用(1251的半衰期为59.6天）；
5.要注意保证所用的Na1251是新鲜的,陈旧制剂的比活性低；
6.酪氨酸的碘化偶尔会对抗原的抗体结合位点有干扰,因此降低其结合能力,但这种情况很少见。
Anti-TP53/FITC 荧光素标记抗P53抗体IgGMulti-class antibodies规格： 0.2ml
PPAR-delta (peroxisome proliferator-activated receptor) D型-过氧化酶活化增生受体(多肽)Multi-class antibodies规格： 0.5mg
拓普西异构酶Ⅰ抗体 Anti-TPⅠ 0.2ml
MPP3 突触相关102抗体 0.2ml
ERC2 CAST1抗体 0.2ml
Rhesus antibody Rh phospho-JAK2(Tyr221) 磷酸化激酶JAK-2抗体 规格 0.1ml
PPAR-delta (peroxisome proliferator-activated receptor) D型-过氧化酶活化增生受体(多肽)Multi-class antibodies规格： 0.5mg
Anti-Tbx21/T-bet/FITC 荧光素标记T介导的转录调节Tbx21抗体IgGMulti-class antibodies规格： 0.2ml
M2-PK ( pyruvate Kinase M2 ) 酸激酶-M2(抗原)Multi-class antibodies规格： 0.5mg
造磷酸酶抗体 Anti-SHP-1 0.2ml
phospho-STAT3 (Tyr705) 磷酸化信号转导和转录激活3抗体 0.1ml
EYA2 眼睛发育缺失EYA2抗体 0.2ml
Rhesus antibody Rh phospho-M-CSF Receptor(Tyr708) 磷酸化巨噬集落刺激受体抗体 规格 0.1ml
M2-PK ( pyruvate Kinase M2 ) 酸激酶-M2(抗原)Multi-class antibodies规格： 0.5mg
ENA human 人上皮诱导中型粘活化Multi-class antibodies规格： 48T
Anti-Phospho-Met (Tyr1349) 磷酸化肝生长受体(原基因)抗体Multi-class antibodies规格： 0.1ml
Rhesus antibody Rh NPR2L/G21 protein G21质抗体 规格 0.2ml
IL-2 ELISA KIT 小鼠白介素2 96T
TSHB 小鼠抗促甲状腺素抗体 0.1ml
C6orf57 6号染色体开放阅读框57抗体 0.2ml
Anti-Phospho-Met (Tyr1349) 磷酸化肝生长受体(原基因)抗体Multi-class antibodies规格： 0.1ml
Salmonella paratyphi a 甲型副伤抗体 0.2ml
phospho-EGFR (Thr678) 磷酸化表皮生长受体抗体 0.1ml
骨保护配体抗体 Anti-OPGL/RANKL 0.1ml
Anti-SMN1/FITC 荧光素标记运动神经元生存1抗体IgGMulti-class antibodies规格： 0.2ml
Rhesus antibody Rh Phospho-NMDAR2B (Tyr1070) 磷酸化受体2B抗体 规格 0.1ml
ChRM2 (Muscarinic Acetylcholine receptor M2) 毒蕈碱型乙酰受体(抗原)Multi-class antibodies规格： 0.5mg
白介素4重组兔单克隆抗体Phospho-HER2 (Tyr1139)/AF555AF555标记的磷酸化HER2受体抗体
RAB11FIP5/AF555AF555标记的Rab11家族相互作用5抗体
FREM1/AF555AF555标记的外基质FREM1抗体
Aquaporin 4 /AF555AF555标记的水通道4单克隆抗体
PADI4/AF555AF555标记的关节相关基因抗体
Phospho-mTOR (Ser2448)/AF555AF555标记的磷酸化雷帕霉素靶抗体
CD28/AF555AF555标记的CD28抗体
C21orf66/AF555AF555标记的21号染色体开放阅读框66抗体
| 产品名称 | 白介素4重组兔单克隆抗体 |
| 浓度 | 1mg/ml |
| 型号 | EY-01K3289 |
英文名称：IL-4
英文别名:Interleukin-4; IL4; IL 4; B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; BCGF 1; BCGF1; Binetrakin; BSF 1; BSF1; HCGF; Hodgkin’s Cell Growth Factor; IA Inducing Factor; IGG1 induction factor; Il4e12; Interleukin 4; Interleukin 4, isoform 1; Interleukin4; Lymphocyte stimulatory factor 1; Macrophage fusion factor; Mast cell growth factor 2; MCGF2; MFF; MGC79402; Pitrakinra; T cell growth factor 2; TCGF2; IL4_HUMAN; B-cell stimulatory factor 1; BSF-1.
交叉反应: Human, Mouse
标记: Unconjugated
抗体来源: Rabbit
抗体类型: Monoclonal
免疫原: Recombinant human IL-4 protein
纯化方法: affinity purified by Protein A
亚型: IgG
性状: Liquid
浓度: 1mg/ml
储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件: Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
背景资料: The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020]
抗体制备过程：
1.材料与试剂
a．提取的动物 Ig
b．弗氏完全佐剂和弗氏不完全佐剂
c．青霉素和链霉素
d．实验动物 兔
e．其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试，查看免疫效果。
5、如果免疫成功，杀死实验活体，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清（无菌采制）
抗体分子标记技术：
（一）原理
当应用化学氧化法时(NaI)遇强氧化剂,离子被氧化为分子,所生成的自由分子可与某些基团进行卤化反应。蛋白质分子可进行卤化反应的基团主要为酪氨酸残基,某些组氨酸残基也可能进行化。在应用胺T( Chloramine T)法的实验中,所用的氧化剂(1,3,4,6- tetrachloro-3a,6adipheny l-glucoluril)强挥发性的有机溶剂中。该溶剂加入试管后,先让其挥发(即让氧化剂将试管包被),然后把Na125I和蛋白质液加入包被好的试管中,反应完成即将混合液移入他管终止反应。
（二）准备
1.试剂
经亲和层析纯化的多克隆抗体或单克隆抗体；0.5mol/L磷酸钠缓冲液,pH7.5；无载体的Na125I3.7GBq/ml(100mCi/ml)的NaOH液；100g/L三醋酸；70%乙醇；新鲜制备的含2mg/m1络胺T的0.5mol/L磷酸钠缓冲液(pH7.5）；胺T反应终止缓冲液；
2.仪器
凝胶过滤柱；￥-记数器；玻璃纤维滤。
（三）操作要点
1.用胺T法进行化,也可在大约pH7.0的缓冲液中进行；
2.如果氧化反应损伤蛋白质，可将抗体与异硫酸荧光素(FITC)偶联)胺T量减少到0.02mg/m，并将偏重亚硫酸液的浓度降到0.024mg/ml；
3. Iodogen-包被的试管可在干燥器中,于室温下保存多年；
4.1251标记的抗体,在制备后六周内均可使用(1251的半衰期为59.6天）；
5.要注意保证所用的Na1251是新鲜的,陈旧制剂的比活性低；
6.酪氨酸的碘化偶尔会对抗原的抗体结合位点有干扰,因此降低其结合能力,但这种情况很少见。
Anti-TP53/FITC 荧光素标记抗P53抗体IgGMulti-class antibodies规格： 0.2ml
PPAR-delta (peroxisome proliferator-activated receptor) D型-过氧化酶活化增生受体(多肽)Multi-class antibodies规格： 0.5mg
拓普西异构酶Ⅰ抗体 Anti-TPⅠ 0.2ml
MPP3 突触相关102抗体 0.2ml
ERC2 CAST1抗体 0.2ml
Rhesus antibody Rh phospho-JAK2(Tyr221) 磷酸化激酶JAK-2抗体 规格 0.1ml
PPAR-delta (peroxisome proliferator-activated receptor) D型-过氧化酶活化增生受体(多肽)Multi-class antibodies规格： 0.5mg
Anti-Tbx21/T-bet/FITC 荧光素标记T介导的转录调节Tbx21抗体IgGMulti-class antibodies规格： 0.2ml
M2-PK ( pyruvate Kinase M2 ) 酸激酶-M2(抗原)Multi-class antibodies规格： 0.5mg
造磷酸酶抗体 Anti-SHP-1 0.2ml
phospho-STAT3 (Tyr705) 磷酸化信号转导和转录激活3抗体 0.1ml
EYA2 眼睛发育缺失EYA2抗体 0.2ml
Rhesus antibody Rh phospho-M-CSF Receptor(Tyr708) 磷酸化巨噬集落刺激受体抗体 规格 0.1ml
M2-PK ( pyruvate Kinase M2 ) 酸激酶-M2(抗原)Multi-class antibodies规格： 0.5mg
ENA human 人上皮诱导中型粘活化Multi-class antibodies规格： 48T
Anti-Phospho-Met (Tyr1349) 磷酸化肝生长受体(原基因)抗体Multi-class antibodies规格： 0.1ml
Rhesus antibody Rh NPR2L/G21 protein G21质抗体 规格 0.2ml
IL-2 ELISA KIT 小鼠白介素2 96T
TSHB 小鼠抗促甲状腺素抗体 0.1ml
C6orf57 6号染色体开放阅读框57抗体 0.2ml
Anti-Phospho-Met (Tyr1349) 磷酸化肝生长受体(原基因)抗体Multi-class antibodies规格： 0.1ml
Salmonella paratyphi a 甲型副伤抗体 0.2ml
phospho-EGFR (Thr678) 磷酸化表皮生长受体抗体 0.1ml
骨保护配体抗体 Anti-OPGL/RANKL 0.1ml
Anti-SMN1/FITC 荧光素标记运动神经元生存1抗体IgGMulti-class antibodies规格： 0.2ml
Rhesus antibody Rh Phospho-NMDAR2B (Tyr1070) 磷酸化受体2B抗体 规格 0.1ml
ChRM2 (Muscarinic Acetylcholine receptor M2) 毒蕈碱型乙酰受体(抗原)Multi-class antibodies规格： 0.5mg
白介素4重组兔单克隆抗体Phospho-HER2 (Tyr1139)/AF555AF555标记的磷酸化HER2受体抗体
RAB11FIP5/AF555AF555标记的Rab11家族相互作用5抗体
FREM1/AF555AF555标记的外基质FREM1抗体
Aquaporin 4 /AF555AF555标记的水通道4单克隆抗体
PADI4/AF555AF555标记的关节相关基因抗体
Phospho-mTOR (Ser2448)/AF555AF555标记的磷酸化雷帕霉素靶抗体
CD28/AF555AF555标记的CD28抗体
C21orf66/AF555AF555标记的21号染色体开放阅读框66抗体
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白介素4重组兔单克隆抗体
￥1080 - 3200
