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文献和实验, D) Lysine (pKa 10), Arginine (pKa 12) 1. Great surface residues: side chain has both aliphatic and ionic character 既亲水(末端正电),又疏水(长碳链) 2. DNA-binding residues;Salt bridges 3. C-terminus of α helices 4. Lys: mobile (great solubilizer): Lys →
from the sample is critical, because salt prevents the peptides from binding to the strong cation-exchange portion of the 2D column. A sample containing 1 M salt will fail in a MuDPIT analysis. In addition, the extraction cartridge concentrates
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples.Moreover,the development of two-dimensional (2D)gel electrophoresis has provided a tool for differential protein display,which allows
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