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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
- 适应物种:
Transfected
- 保质期:
12个月
- 库存:
现货
- 宿主:
Mouse
- 应用范围:
WB
- 靶点:
Maltose Binding Protein
- 规格:
50μL/100μL
| 规格: | 50μL | 产品价格: | ¥1400.0 |
|---|---|---|---|
| 规格: | 100μL | 产品价格: | ¥2400.0 |
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文献和实验Maltose-Binding Protein as a Solubility Enhancer
as a general solubilizing agent, this has not turned out to be the case. In a direct comparison with glutathione S-transferase (GST) and thioredoxin, maltose-binding protein (MBP) was decidedly superior at solubilizing a diverse collection of aggregation-prone
Purification of MBP (maLTose-binding proteins) Fused Proteins
with 10 fractions of 10 mM maltose in 20 mM Tris pH 7.4, 200 mM NaCl, 10 mM b ME, 1 mM EDTA or in some other buffer of choice, depending on subsequent purification steps. If the fusion protein is to be cleaved with factor Xa as the next step
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
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