In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ROS Measurement:1. Incubate the cells according to your normal protocol.2. Treat cells with 10 μM CM-H2DCFDA for 30 min at 37°C in darkness.3. Wash the excess probe.4. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.