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- 文献和实验
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- 保存条件:
4°C, protect from light
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
850013-49-9
- 规格:
50 μg
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CM-H2DCFDA
CAS No. : 850013-49-9
MCE 国际站:CM-H2DCFDA
产品活性:CM-H2DCFDA 是 H2DCFDA (HY-D0940) 的衍生物。CM-H2DCFDA 可用于测定细胞氧化水平 (Ex/Em: 495/530 nm)。CM-H2DCFDA 对光敏感。
研究领域:Immunology/Inflammation | NF-κB | Metabolic Enzyme/Protease
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ROS Measurement:1. Incubate the cells according to your normal protocol.2. Treat cells with 10 μM CM-H2DCFDA for 30 min at 37°C in darkness.3. Wash the excess probe.4. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
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文献和实验Blue Native Gel Electrophoresis
Blue Native Gel Electrophoresis Stock solutions 49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT 3 x Gel buffer 150 mM BisTris-HCl, 1.5 M 6-amino-caproic acid, pH 7.0 Adjust pH to 7.0 with HCl at 4°C
GuSCN 加少量双蒸水溶解,然后加入其余成分,65 ℃ 加热使其充分溶解,4 ℃ 保存备用。(2)GuSCN/25 mmol/L β-巯基乙醇溶液:将 0.36 mL β-巯基乙醇溶于 200 mL GuSCN 缓冲中,4 ℃ 保存备用。(3)2M NaAc (pH 4.0)(4)0.1% DEPC-H2O:将 0.1 mL DEPC 加入 100 mL 双蒸水中,室温摇晃 12~24 小时,然后 15 磅高压 15 min(注意:若用此水处理容器,可以不用高压处理)(5)氯仿: 异戊醇 (49
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
) (optional, see Step 33) Ethanol (95%) Fixing solution Giemsa (Merck 109204) Glycerol (99.5%) H2 O2 (Merck 107210) Hoechst staining solution Liquid nitrogen Methanol (optional, see Step 23) Mowiol-DABCO
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