Biotinylated Recombinant Human GPC3/Glypican 3 Protein, His-Avi Tag
CAS号:
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保质期:
一年
供应商:
翌圣生物科技(上海)股份有限公司
保存条件:
-20度
规格:
100ug
产品描述 Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6).Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed. 产品性质
Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln25-His559.
Molecular Weight
The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 80-135 kDa based on Tris-Bis PAGE result.
Purity
> 90% as determined by Tris-Bis PAGE
Endotoxin
< 1.0EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
运输与保存方法 The products are shipped with ice pack and can be stored at -20℃for 1 year. 2-7 days, 2 to 8 °C under sterile conditions after reconstitution. 3 -6 months, -20 to -80°C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. 注意事项1.For your safety and health, please wear lab coats and disposable gloves for operation. 2.For research use only!