- 询价
- 近岸蛋白(Novoprotein)
- 中国
- EB206
- 2025年10月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃保存。
- 保质期：
1 year
- 供应商：
近岸蛋白
- 规格：
EB206-01A 500ml
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
by baking for 1 -2 hours at 80°C in a vacuum oven. Any filters not used immediatley in hybridization reaction should be wrapped loosely in aluminum foil and stored at room temperature. Time Required: 2 - 3 hours 2. Labeling of DNA-Probe
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
analysis of genomic DNA by southern blotting
prior to removing the probe, as drying will cause the probe to bind irreversibly. To remove probe from blot for reuse. incubate one of the following solutions: Wash blots in 50% formamide, 6 x SSPE for 30 minutes at 65°C. ( for 50 ml: 25 ml
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