In Vitro: Manzamine A (5-50 µM, 18 h) decreases tau phosphorylation, measured with ELISA. Manzamine A (10 µM) inhibits yeast S. cerevisiae growth by 30%. Manzamine A displays a few enlarged vacuoles in yeast. Manzamine A (2.5-10 µM, 24 h) increases acidity in pancreatic cancer cells and non-malignant Vero cells. Manzamine A (1 µM, 24 h) inhibits HSV-1 infection in SIRC cells. Manzamine A shows antimalarial activity with an IC50 of 8.0 nM (D6 clone) and 11 nM (W2 clone).
In Vivo: Manzamine A (50 and 100 mol/kg, p.o. or i.p.) inhibits the growth of the rodent malaria parasite Plasmodium berghei in infected mice. Manzamine A (8 mg/kg, i.p., daily for 8 consecutive days) prolongs the survival of SW mice to 20 days.