Gol-NTR,2968461-58-5

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Gol-NTR

CAS No. :

MCE 国际站：Gol-NTR

产品活性：Gol-NTR 是一种高选择性和高灵敏度的高尔基体靶向探针。Gol-NTR 是硝基还原酶 (NTR) 激活的，具有可视化急性肺损伤 (ALI) 和修复功能。Gol-NTR 具有 54.8 ng/mL 的低检测限。Gol-NTR 可用于监测和评估脓毒症诱导的 ALI 研究反应的研究。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: Fluorescent labeling of NTR by Gol-NTR
(1) Prepare 1.0 mM Gol-NTR stock solution with DMSO solution.
(2) Dilute the stock solution with DMSO solution to prepare 5.0 μM Gol-NTR working solution.
(3) Mix 5.0 μM Gol-NTR with 50 μM NADH in PBS buffer (10 mM, pH 7.4) containing 5% DMSO, and then add appropriate NTR.
(4) After incubation at 37℃ for 30 min, the spectra was recorded at 405 nm (slit width: d_ex/d_em=5/5 nm).
Fluorescence labeling of NTR in A549 cells by Gol-NTR
(1) A549 cells were cultured at different oxygen concentrations (1%, 5%, 10%, 15% and 20% O₂) for 8 h.
(2) A549 cells were washed with phosphate buffered saline (PBS).
(3) A549 cells were treated with 5.0 μM Gol-NTR for 1 h.
(4) Fluorescence images of A549 cells were observed using confocal fluorescence imaging.

In Vivo: In Vivo Imaging
(1) C57BL/6 male mice (6-8 weeks old, weight 20-22 g) were pre injected with 300 μL DMOG (25 mg/mL), after 24 h, intraperitoneal injection of 300 μL LPS (10 mg/kg) for 6 h.
(2) Mice were killed by cervical vertebra dislocation and lung organs were collected.
(3) After washing with PBS, incubate with 50 μM Gol-NTR in PBS for 1 h.
(4) After washing with PBS, fluorescence imaging was performed on a small animal imaging system (excitation wavelength of 420 nm and emission wavelength of 510 nm).

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