In Vitro: ML-SI3 (10 μM) inhibits ML-SA1-evoked Ca2+ signals in HeLa cells. ML-SI3 (25-75 μM, 24h) disrupts tegumental integrity of adult schistosomes. ML-SI3 (10 μM) blocks Rapamycin (HY-10219)-evoked ITRPML1 in mimic of lysosomal lumen. ML-SI3 (3 µM, 6 h) abolishes the increase in both LC3II and p62 levels induced by hypoxia/reoxygenation (H/R) (4 h H/2 h R) in neonatal rat ventricular myocytes (NRVM).
In Vivo: ML-SI3 (1.5 mg/kg, i.p., four times) attenuates I/R injury in mouse cardiomyocytes.