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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
零下80~20°C
- 保质期:
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名:
Gel filtration medium G-100
- 库存:
10
- 供应商:
TargetMol
- CAS号:
9050-94-6
- 规格:
25 mg
Product Introduction
Bioactivity
英文名:Gel filtration medium G-100
描述:Gel filtration medium G-100, a substance designed for protein purification, enables the separation and purification of proteins through gel filtration.

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文献和实验具有三维网状结构的凝胶颗粒不能使比其网眼大的分子进入,但可使比其小的分子渗入内部,渗入程度因分子的大小而异。因此,高分子溶液在通过凝胶柱时,一定大的分子便能很迅速地通过而被滤出。而大小在其以下的分子可依次缓慢地滤出。应用这种原理,按照分子的大小将分子分部过滤的技术称为凝胶过滤或凝胶柱层析。此法由J. Porath等( 1969)所创造。通常使用的凝胶是葡聚糖凝胶、琼脂糖凝胶、聚丙烯酰胺,并可选择桥接度适宜的凝胶。凝胶过滤法被广泛地用于生物体高分子的分部、精制、脱盐、缓冲液交换
Native RNA Purification by Gel Filtration Chromatography
. Herein, we describe the native isolation of RNA molecules by FPLC purification using Superdex 75 or 200 gel filtration columns. This approach can be extended to purify biologically interesting RNA complexes such as RNA–protein complexes that have been generated
Thin-Layer Gel Filtration: Determination of Protein Molecular Weights
For many years Sephadex gel filtration of proteins has been a valuable tool for separating proteins on the basis of size difference. Sephadex is a bead-formed gel prepared by crosslinking dextran, and is available in a variety of G-types
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