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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
零下80~20°C
- 保质期:
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名:
DGN549-C
- 库存:
10
- 供应商:
TargetMol
- CAS号:
2058075-34-4
- 规格:
25 mg
Product Introduction
Bioactivity
英文名:DGN549-C
描述:DGN549-C is a novel compound featuring a cleavable ADC (antibody-drug conjugate) linker composed of valine-alanine (va) and a PBD (pyrrolobenzodiazepine) dimer. This compound possesses a unique DNA-alkylating cytotoxic payload, DGN549, which is utilized in the development of ADCs.

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文献和实验Construction and Characterization of Adenovirus Vectors
C. Cells: A549 Cells: 293, low-passage Grow 293 and A549 cells in 150-mm dishes in a 37°C, 5% CO2 incubator and split 1:2 or 1:3 when they reach ~90% confluence. Cells: 293N3S Maintain 293N3S cells in 150-mm dishes
细胞介导的细胞毒性和抗体依赖的细胞介导的细胞毒性(ADCC)试验中灵活选择效应细胞和靶细胞 3. 可采用3D肿瘤球模型,对免疫细胞杀伤进行实时查看和量化 图6 将PBMC与SKOV-3(HER2阳性)或A549(HER2阴性)肿瘤细胞共培养,其中肿瘤细胞预先用NucLight Red荧光试剂标记。(A) 曲妥珠单抗诱导SKOV3肿瘤细胞增殖呈浓度依赖性抑制效应,(B) A549细胞未观察到抑制作用。(C) 曲妥珠单抗抑制SKOV-3细胞增殖的浓度反应曲线。(D) 对照组和曲妥昔单抗组在72小时
to 1.0 or 1.5 ml YPD/15% glycerol in a Sarstedt tube. Transfer enough yeast into the tube to get an OD600 of about 5 (estimate by eye). Freeze at -70° C before cells the settle. One 100 place box should be used per background per 96-pack
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