重组新冠病毒 (BA.2.75)Spike RBD 蛋白 (His&AVI 标签),生物素标记

重组新冠病毒 (BA.2.75)Spike RBD 蛋白 (His&AVI 标签),生物素标记（产品说明）
蛋白名称：SARS-CoV-2 BA.2.75 (Omicron) Spike RBD Protein (His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (BA.2.75) Spike RBD (YP_009724390.1, with mutation G339H, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.75). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
蛋白活性：Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.2.75)Spike RBD Protein (His&AVI Tag),Biotinylated(Cat: 40592-V49H10-B), the EC50 is 1.8-6 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 Spike RBD consists of 249 amino acids and predicts a molecular mass of 28.56 kDa. As a result of glycosylation, it migrates as an approximately 34.72 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541
蛋白标签：C-His-AVI
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.