Detects a band of approximately 14,16 kD(Predicted molecular weight: 14 kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
免疫原
Purified recombinant protein expressed in E.coli.
性能
形式
Monoclonal Antibody
纯化方法
Affinity Purified
克隆号
8F5
同种型
IgG1
储存/保存方法
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
存储溶液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
修饰
Unmodified
靶标
背景说明
Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
UniProt
Q9H492
实验结果图:
Western blot analysis of LC3A (8F5)in Hela lysates, 3T3 lysates, Rat Brain lysates using LC3A antibody.
Immunohistochemistry analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunohistochemistry analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A antibody.