- ￥1500 - 2300
- 爱必信(absin)
- 上海
- abs120224
- 2025年07月13日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated synthetic peptide derived from human IL-1 Beta:161-269/269
- 亚型：
-
- 形态：
-
- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 克隆性：
-
- 标记物：
-
- 适应物种：
Human, Mouse, Rat
- 保质期：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 抗原来源：
-
- 目录编号：
-
- 级别：
-
- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 靶点：
-
- 抗体英文名：
-
- 抗体名：
IL-1 β抗体
- 规格：
100ul/50ul
| 规格： | 100ul | 产品价格： | ￥2300.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1500.0 |
| 别名 | IL-1 β抗体;Catabolin, H1, IL-1β, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL1B, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta, IL1B_HUMAN, Interleukin-1 beta, IL-1 beta. , ,IL-1B |
| 宿主 | Rabbit |
| 特异性 | IL-1 Beta |
| 反应种属 | Human;Mouse;Rat; |
| 预测反应种属 | Dog;Pig;Rabbit;Guinea Pig; |
| 应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复） |
| 分子量 | Predicted molecular weight: 17kD/32kDa Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 | KLH conjugated synthetic peptide derived from human IL-1beta:101-200/269 |
| 浓度 | 1mg/ml |
| 纯化方法 | affinity purified by Protein A |
| 类型 | Polyclonal Antibody |
| 同种型 | IgG |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 研究领域 | 细胞生物 免疫学 糖蛋白 |
| 背景说明 | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]. |
| 细胞定位 | 分泌型蛋白 |
| UniProt | P01584 |
Sample: Raw246.7 (Mouse)Cell Lysate at 40 ug
Primary: Anti- IL-1 beta (abs120224) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 30 kD
Tissue/cell: Human cervical carcinoma tissue; 4% PA-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Tissue/cell: rat brain tissue; 4% PA-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Blank control (black line): A431(black) (The cells were fixed with 2% PA (10 min) , then permeabilized with PBST for 30 min on room temperature)
Primary antibody: Rabbit Anti-PTP1B antibody ;Dilution: 1μg /10^6 cells;
Isotype Control antibody: Rabbit IgG .
Secondary antibody: Goat anti-rabbit IgG-FITC;Dilution: 1μg /test.
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- 作者
- 内容
- 询问日期
文献和实验- Angiotensin Type 2 Receptor Pharmacological Agonist Relieves Neurocognitive Deficits via Reducing Neuroinflammation and Microglial Engulfment of Dendritic SpinesShen, Liang, Chen, Dan-yang, Lou, Qian-qian, Cao, Peng, Hu, Rui, Jin, Yan, Wang, Di, Hu, Shan-shan
Journal of Neuroimmune Pharmacology. 2022 Dec 05 .
影响因子： 7.285
- Kinsenoside Alleviates 17α-Ethinylestradiol-Induced Cholestatic Liver Injury in Rats by Inhibiting Inflammatory Responses and Regulating FXR-Mediated Bile Acid HomeostasisJiaxiong Ming,Qianqian Xu,Limin Gao,Yanfang Deng,Jie Yin,Qun Zhou,Qingyi Tong ,Yonghui Zhang
Pharmaceuticals. 2021 May 11 ; 5
影响因子： 5.68
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
suspended in PBS. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600
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