保存方法:Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
描述:
FLI-06 is a Notch inhibitor and the early secretory pathway inhibitor. FLI-06 disrupts the Golgi apparatus in a manner distinct from that of brefeldin A and golgicide A.
In HeLa NotchΔE-eGFP cells, FLI-06 blocks Notch trafficking and processing. FLI-06 changes the maturation pattern of APP and abolishes the shedding of APPs in HEK293 cells stably expressing a mutated APP that yields robust amounts of amyloid β. FLI-06 disrupt the Golgi apparatus, and inhibits general secretion at a step before exit from the endoplasmic reticulum (ER), which is accompanied by a tubule-to-sheet morphological transition of the ER.
体内研究:FLI-06 (50 μM) inhibits endogenous Notch signaling in zebrafish embryos.