2-Oleoyl Glycerol-d5,946524-37

Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosol

modified D1R and D5R cDNAs are prepared using a polymerase chain reaction method, propagated in E. coli , purified and mutations confirmed by DNA sequencing. Receptor expression constructs are transfected into HEK293 cells cultured in vitro at 37�C

Preparing Overnight Bacterial Culture

stir to dissolve pH to 7.0 autoclave to sterilize cool to 50-60 degrees C add antibiotic if needed Agar plate with bacterial colonies: scrape bacteria glycerol stock

Formation of Pit-Spanning Phospholipid Bilayers on Nanostructured Silicon Dioxide Surfaces for Studying Biological Membrane Events

adsorption, which under certain conditions may be exploited to form a suspended bilayer. Here we describe the use of nanostructured SiO2 surfaces prepared by the colloidal lithography technique to scrutinize the formation of suspended 1-palmitoyl-2-oleoyl-sn