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- 保存条件：
运输及储存于4℃条件下，保质期6个月。
- 库存：
99
- 供应商：
爱必信(上海)生物科技有限公司
- 规格：
96T
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产品描述： 产品名称：NADH Dehydrogenase Microplate Assay Kit 中文别名: NADH-辅酶Q还原酶(复合体I), EC 1.6.5.3, ubiquinone reductase (ambiguous), type 1 dehydrogenase, complex 1 dehydrogenase, coenzyme Q reductase (ambiguous), complex I (electron transport chain), complex I (mitochondrial electron transport), complex I (NADH:Q1 oxidoreductase), dihydronicotinamide adenine dinucleotide-coenzyme Q reductase (ambiguous), DPNH-coenzyme Q reductase (ambiguous), DPNH-ubiquinone reductase (ambiguous), mitochondrial electron transport complex 1, mitochondrial electron transport complex I, NADH coenzyme Q1 reductase, NADH-coenzyme Q oxidoreductase (ambiguous), NADH-coenzyme Q reductase (ambiguous), NADH-CoQ oxidoreductase (ambiguous), NADH-CoQ reductase (ambiguous), NADH-ubiquinone reductase (ambiguous), NADH-ubiquinone oxidoreductase (ambiguous), NADH-ubiquinone-1 reductase, reduced nicotinamide adenine dinucleotide-coenzyme Q reductase (ambiguous), NADH:ubiquinone oxidoreductase complex, NADH-Q6 oxidoreductase (ambiguous), electron transfer complex I, NADH2 dehydrogenase (ubiquinone) 储存/保存方法: 运输及储存于4℃条件下，保质期6个月。 应用: 用于检测及定量NADH Dehydrogenase的活性. 用途说明: 组织提取物, 细胞裂解液, 细胞培养上清, 其他生物液体样本 检测类型: 比色法 检测范围: 4 umol/L - 400 umol/L
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文献和实验Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements
of standardization and reproducibility, lack of technical robustness, and poor methodological efficacy. Among these, the microplate adenosine triphosphate (ATP)-based tumor chemosensitivity assay (ATP-TCA) has gained particular merits for ex vivo chemosensitivity
, first described by Mosmann in 1983, is based on the ability of a mitochondrial dehydrogenase enzyme (by generating reducing equivalents such as NADH and NADPH) from viable cells to cleave the tetrazolium rings of the pale yellow MTT and form a dark blue formazan
The MTT Cell Proliferation Assay
proliferation. The yellow tetrazolium MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by metabolically active cells, in part by the action of dehydrogenase enzymes, to generate reducing equivalents such as NADH and NADPH
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