8668 ScienCell 比色法组蛋白乙酰转移酶活性测定, Colorimetric Histone Acetyltransferase Activity Assay,产品说明: 组蛋白乙酰转移酶(HAT)通过从辅因子乙酰辅酶A转移乙酰基来催化组蛋白的乙酰化,从而调节基因表达。该调节对于细胞分化和增殖等细胞过程至关重要。我们的比色组蛋白乙酰转移酶(HAT)活性检测试剂盒可快速灵敏地检测哺乳动物细胞/组织样品中的HAT活性或HAT抑制。简而言之,HATs对肽底物的乙酰化释放出游离形式的CoA,其可以作为NADH生成酶的辅酶来产生NADH。化学计量NADH的产生可以通过与可溶性四唑染料水溶性四唑-1(WST-1)反应时在440nm处的分光光度分析来量化。HAT测定不需要放射性或色谱,可用于动力学研究。HAT的反作用酶组蛋白脱乙酰酶(HDACs)不会干扰测定,因此可以使用粗核提取物或细胞裂解物样品。 Histone acetyltransferases (HATs) catalyze the acetylation of histone proteins by transferring an acetyl group from cofactor acetyl-CoA, and consequently regulate gene expression. The regulation is critical for cellular processes such as cell differentiation and proliferation. Our Colorimetric Histone Acetyltransferase (HAT) Activity Assay Kit offers rapid and sensitive detection of HAT activity or HAT inhibition in mammalian cells/tissue samples. Briefly, acetylation of a peptide substrate by HATs releases the free form of CoA, which can serve as a coenzyme of a NADH generating enzyme to produce NADH. The stoichiometric NADH production can be quantified by spectrophotometric analysis at 440 nm upon reacting with water soluble tetrazolium-1 (WST-1), a soluble tetrazolium dye. The HAT assay does not require radioactivity or chromatography and can be applied for kinetic studies. HATs' counteracting enzymes, histone deacetylases (HDACs), do not interfere with the assay, consequently crude nuclear extract or cell lysate samples can be used.