8578 ScienCell 醛胶氢酶检测试剂盒 ALDH, Aldehyde Dehydrogenase Assay,产品说明: 醛脱氢酶(ALDH)是催化各种醛转化为相应羧酸的酶家族。ALDH 在酒精代谢中起重要作用,将具有致癌、细胞毒性和致突变性的乙醛转化为乙酸。ALDH 活性和表达水平的变化可用作癌症、癌症干细胞和帕金森病的标志物。该比色测定基于醛脱氢酶催化的醛氧化,其中产生的NADH可以将几乎无色的探针转化为有色产品;有色产品的强度与样品中ALDH的量成正比,在440nm处表现出最大的吸光度。 Aldehyde dehydrogenase (ALDH) is a family of enzymes that catalyze the conversion of various aldehydes to their corresponding carboxyolic acids. ALDH plays an important role in alcohol metabolism, converting acetaldehyde, which has carcinogenic, cytotoxicity, and mutagenicity affects, into acetic acid. The change in ALDH activity and expression levels can be used as a marker for cancer, cancer stem cells, and Parkinson’s disease. This colorimetric assay is based on aldehyde dehydrogenase-catalyzed oxidation of aldehyde, where the resulting NADH can then convert a nearly colorless probe to a colored product; the intensity of the colored product is proportional to the amount of ALDH in the sample, exhibiting maximum absorbance at 440nm.