8568 ScienCell 乙*脱氢酶活性检测试剂盒 ADH, Alcohol Dehydrogenase Assay,产品说明: 酒精脱氢酶(ADH)包括一系列酶,可催化许多生物体中酒精转化为醛。ADH在酒精解*中起重要作用,并导致致癌乙醛的产生,乙醛可以通过醛脱氢酶进一步转化为乙酸。血清中 ADH 活性的测量可能是肝损伤和肝炎的敏感指标。该比色测定基于乙*脱氢酶催化乙*氧化,其中形成的NADH可以将几乎无色的探针转化为颜色强烈的产物,其在440nm处表现出最大吸光度,这与样品中的ADH量成正比。 Alcohol dehydrogenase (ADH) comprises a family of enzymes that catalyzes the conversion of alcohol to aldehydes in many organisms. ADH plays an important role in alcohol detoxification and leads to the generation of carcinogenic acetaldehyde which can be further converted into acetic acid by aldehyde dehydrogenase. The measurement of ADH activity in serum can be a sensitive indicator of hepatic injury and hepatitisis. This colorimetric assay is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH can convert a nearly colorless probe to an intensely colored product, which exhibits maximum absorbance at 440nm, which is proportional to the amount of ADH in the sample.