谷胱甘肽S-转移酶(GST)包括一系列酶,在异生素的细胞解*中起重要作用。GST催化还原型谷胱甘肽与亲电异生素的结合,并利用还原型谷胱甘肽清除潜在的有*化合物。GST活性测量已被用作诊断肿瘤和肝脏损伤以及氧化应激的生物标志物。该比色测定基于还原型谷胱甘肽和GST底物1-*-2,4-二*基*(CDNB)之间的GST催化反应。GST活性是通过测量还原型谷胱甘肽和CDNB之间产生的偶联速率来确定的,该速率与340nm处吸光度随时间的增加(ΔOD340nm / min)成正比。 Glutathione S-transferases (GST) comprise a family of enzymes that plays an important role in cellular detoxification of xenobiotics. GST catalyzes the conjugation of reduced glutathione to electrophilic xenobiotics and utilizes reduced glutathione to scavenge potentially toxic compounds. GST activity measurement has been used as biomarker for diagnoses of tumor and liver damage and oxidative stress. This colorimetric assay is based on the GST-catalyzed reaction between reduced glutathione and GST substrate, 1-chloro-2, 4-dinitrobenzene (CDNB). The GST activity is determined by measuring the rate of produced conjugation between reduced glutathione and CDNB, which is proportional to the increase in absorbance at 340nm over time (ΔOD340nm/min).