8468 ScienCell 甘油检测试剂盒 GLY, Glycerol Assay,产品说明: 甘油是肝脏和脂肪组织中合成甘油三酯和磷脂的前体。在一些生物体中,甘油可以被肝脏转化为葡萄糖,并为细胞代谢提供能量。循环甘油的测量被认为反映了脂肪分解,因此是在不同条件下评估的有用参数。该比色测定基于甘油的偶联酶促反应,其中形成的过氧化*由过氧化物酶催化并与4-*基安替比林反应形成产物染料。反应产物在540nm处的颜色强度与样品中的甘油水平成正比。 Glycerol is a precursor for synthesis of triglycerides and of phospholipids in the liver and adipose tissue. In some organisms, glycerol can be converted into glucose by the liver and provide energy for cellular metabolism. The measurements of circulating glycerol are considered to reflect lipolysis and are therefore useful parameters to evaluate in different conditions. This colorimetric assay is based on a coupled enzymatic reaction of glycerol, in which the formed hydrogen peroxide is catalyzed by peroxidase and reacts with 4-aminoantipyrine to form the product dye. The color intensity of the reaction product at 540nm is directly proportional to glycerol level in the sample.