8398 ScienCell 葡萄糖6磷酸酶检测试剂盒 G6P , Glucose-6-phosphate Assay,产品说明: 葡萄糖-6-磷酸(G6P)是糖酵解、己糖单磷酸分流以及糖原和淀粉生物合成途径中的第一个底物。G6P被葡萄糖-6-磷酸脱氢酶利用,以NAD(P)H的形式产生还原当量。这在红细胞中尤其重要,其中G6PDH缺乏导致溶血性贫血。该比色测定基于葡萄糖-6-磷酸脱氢酶催化G6P的氧化,其中形成的NADPH可以将几乎无色的探针转化为颜色强烈的产物,其在440nm处表现出最大吸光度,与样品中G6P的量成正比。 Glucose-6-phosphate (G6P) is the first substrate in glycolysis, the hexose monophosphate shunt and both the glycogen and starch biosynthesis pathways. G6P is utilized by glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. This colorimetric assay is based on glucose-6-phosphate dehydrogenase catalyzed oxidation of G6P, in which the formed NADPH can convert a nearly colorless probe to an intensely colored product, which exhibits maximum absorbance at 440nm, is proportional to the amount of G6P in the sample.