Pluripotent stem cells (PSCs) have the unique capability to proliferate indefinitely and to differentiate into all cell types. Due to their self-renewal property and pluripotency, PSCs have become a promising candidate for therapeutic applications. Elevated level of alkaline phosphatase expression is one of the most widely used stem cell markers. ScienCell™ Alkaline Phosphatase Activity Assay is optimized to detect alkaline phosphatase activity in PSCs and provide a quantitative assay for measuring stem cell undifferentiation/differentiation.