8008 ScienCellI型胶原细胞粘附检测试剂盒,Collagen I Cell Adhesion AssayKit,产品说明: 细胞粘附在细胞通讯和调节中起重要作用,在组织的发育和维持中至关重要。胶原蛋白I是细胞外基质的主要结构成分,是培养许多不同细胞类型的jijia底物。ScienCell 的胶原蛋白 I 细胞粘附检测专为快速、定量和可靠地测量细胞对胶原蛋白 I 的粘附而设计。该试剂盒包括预涂有胶原蛋白 I 的 48 孔板,以及作为阴性对照的 BSA(牛血清白蛋白)(参见图 1 中的板格式)。将细胞在预包被的孔中培养所需时间,然后冲洗掉未结合的细胞,固定粘附的细胞并进行染色,然后进行提取步骤,该步骤导致染料从染色细胞洗脱成上清液。因此,可以使用比色法ELISA酶标仪在595nm处定量细胞粘附。
Cell adhesion plays an important role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Collagen I, a major structural component of extracellular matrices, is an excellent substrate for the culture of many different cell types. The ScienCell' Collagen I Cell Adhesion Assay is designed for the rapid, quantitative and reliable measurement of cell adhesion to collagen I. The kit includes a 48-well plate pre-coated with collagen I, as well as BSA (bovine serum albumin) as negative controls (see plate format in Figure 1). Cells are cultured in the pre-coated wells for a desired period of time, then unbounded cells are washed away, and the adhered cells are fixed and stained, followed by an extraction step which leads to dye elution from stained cells into supernatant. Thus cell adhesion can be quantified using a colorimetric ELISA plate reader at 595 nm.