一抗—包括单克隆抗体和多克隆抗体 多克隆抗体优势识别多个抗原表位,这意味着:1. 多抗能放大弱表达蛋白的信号,因为多个抗原表位结合了多个抗体分子(不利于定量实验)。2. 更能容忍抗原的微小变化(如多态性、糖基化的异质性,或细微的变性)。3. 能结合与免疫原高度同源的蛋白,或被用来筛选不同种属来源的靶标蛋白。劣势1. 容易产生批次间差异2. 不利于检测抗原的特殊结构域。 单克隆抗体 优势识别一个抗原表位,这意味着:1. 特异检测一个特殊/确定的抗原表位(与其他蛋白的交叉反应可能性小)。2. 一致性高,意味着当实验条件恒定时,实验结果的可重复性非常高。劣势1. 其它种属来源的靶标蛋白可能不含抗原表位。27. 一个抗原表位更容易因化学处理而丢失(可以使用两种或两种以上识别同一抗原的单抗)。反应种属:Human;Mouse;Rat克隆性:Polyclonal Antibody保存方法:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles产品描述:murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr731 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.