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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Phospho-AKT1 (Ser473) Monoclonal Antibody_货号:E-AB-51038_修饰性抗体
- 抗体英文名：
Phospho-AKT1 (Ser473) Monoclonal Antibody
- 应用范围：
WB;IHC-p
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12个月
- 目录编号：
E-AB-51038
- 级别：
科研级别
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
- 形态：
液体
- 亚型：
IgG
- 规格：
120μL/60μL/20μL
| 规格： | 120μL | 产品价格： | ￥2415.0 |
|---|---|---|---|
| 规格： | 60μL | 产品价格： | ￥1510.0 |
| 规格： | 20μL | 产品价格： | ￥630.0 |
货号：E-AB-51038
| Verified Samples |
Verified Samples in WB:PC-3
Verified Samples in IHC:Human lung carcinoma |
| Dilution |
WB 1:1000-2000, IHC 1:100-200
|
| Clonality |
Monoclonal
|
| Isotype |
IgG
|
| Immunogen |
Synthetic Peptide of Phospho-Akt (S473)
|
| Abbre |
Phospho-AKT1 (Ser473)
|
| Synonyms |
AKT 1;AKT;AKT1;AKT1;MGC99656;PKB;PKB-ALPHA;PRKBA;Protein Kinase B Alpha;Protein kinase B;Proto-oncogene c-Akt;RAC Alpha;RAC;RAC-alpha serine/threonine-protein kinase;RAC-PK-alpha抗体
|
| Swissprot |
P31749
|
| Observed MW |
60kDa
|
| Cellular Localization |
Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.
|
| Tissue Specificity |
Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
|
| Concentration |
1 mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
| Buffer |
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
|
| Purification Method |
Protein A purification
|
| Research Areas |
Cancer; Epigenetics and Nuclear Signaling; Metabolism; Signal Transduction
|
| Clone No. |
Clone:7F9
|
| Conjugation |
Unconjugated
|
| Background |
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
|
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【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体？
对照，2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R]（货号#E022030），其免疫原是KLH偶联的水母GFP蛋白的N末端多肽，由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致，因此从免疫原上来说，GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是，EarthOx对于该单克隆抗体还进行了明确的验证试验，试验也表明该Anti-GFP Tag Monoclonal
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
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