Grace's Unsupplemented Insect Medium does not contain lactalbumin hydrolysate or yeastolate, commonly found in the supplemented version. Gibco™ Grace's Unsupplemented Insect Medium is also made without methionine for protein labeling studies. Product Use: For Research Use Only: Not intended for animal or human diagnostic or therapeutic use. cGMP Manufacturing and Quality System: Gibco™ Grace's Unsupplemented Insect Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Grace's Unsupplemented Insect Medium requires supplementation with 10% Fetal Bovine Serum (FBS), 50 mg/L methionine, 3.33 g/L lactalbumin hydrolysate and 3.33 g/L yeastolate before use as a growth medium. Grace's Unsupplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.