与其他基础培养基相比,F-12 含有更广泛的成分,包括锌、腐胺、次黄嘌呤和胸苷。F-12 中 CHO 细胞的无血清生长已产生多种改进配方。
cGMP 生产和质量体系 Gibco™ F-12 在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 注册的医疗器械生产商,且通过 ISO 13485 标准认证。
Gibco™ F-12 不含蛋白或生长因子。因此,F-12 需要添加剂,通常采用 10%胎牛血清 (FBS)。F-12 使用碳酸氢钠缓冲系统 (1.176 g/L),所以需要 5-10 % CO2 环境来维持生理 pH 值。粉末形式的 Gibco™ 细胞培养基在配制时需要补充碳酸氢钠、调节 pH 并过滤
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. cGMP Manufacturing and Quality System: Gibco™ F-12 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation