sterility:sterile-filtered.form:liquid.technique(s):cell culture | insect: suitable.components:L-glutamine: 0.6 g/L;glucose: 10 g/L (Dextro).Application:Shields and Sang M3 Insect Medium has been used to culture Drosophila cell lines, larvae, and testes. Developed for the culture of Drosophila cell lines.Biochem/physiol Actions:Insect tissue culture media have been formulated to mimic the main properties of the hemolymph of specific insects. A survey of the formulas of these media reveals significant differences in their composition. When supplemented with 5-20% heat-inactivated fetal bovine serum, Shields and Sang M3 medium has been found to support the rapid growth of both primary and established cultures of Drosophila melanogaster cells.