5901 ScienCell人多能干细胞向心肌细胞分化试剂盒

ScienCell 研究实验室（ScienCell）是一家生物技术公司，其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞，细胞培养基和试剂，培养基添加因子，细胞来源的RNA，cDNA和蛋白质。ScienCell的科学家们以改善生活质量，实现细胞的能力，打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥，公司成立于1999年。



5901 ScienCell人多能干细胞向心肌细胞分化试剂盒PSCCDK，Human Pluripotent StemCell Cardiomyocyte Differentiation Kit, 50 ml，产品简介：
产品品牌: ScienCell
产品货号：5901
产品名称：5901 ScienCell人多能干细胞向心肌细胞分化试剂盒PSCCDK，Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit, 50 ml
产品规格：50 ml

多能干细胞可以来源于囊胚内细胞团（早期胚胎），称为胚胎干（ES）细胞，也可以由体细胞经转录因子（包括Oct3/4，SOX2，KLF4和c-myc基因）诱导而来，称为多能干（iPS）细胞。这些细胞能够自我更新、分化和衍化成三个胚层：外胚层，中胚层和内胚层。 此外，间充质干细胞是多能干细胞，可以分化成多种类型的细胞，包括成骨细胞、软骨细胞和脂肪细胞。 ScienCell为您的干细胞研究提供优质的产品，包括培养基、生长因子等其它工具。

5901 ScienCell人多能干细胞向心肌细胞分化试剂盒PSCCDK，Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit, 50 ml，产品说明：
人类多能干细胞（hPSC），包括胚胎干细胞（ESC）和诱导多能干电池（iPSC），具有分化为三个生殖层的所有衍生物的能力。鉴于成人心肌细胞通常不增殖，hPSC可能提供无限量的心肌细胞。hPSC衍生的心肌细胞可用于药物筛选、毒性试验和发育研究。
hPSC的自发心脏分化效率低下，培养物中存在的心肌细胞不足1%。通过添加生长因子（BMP4、激活素A和FGF2等）、小分子或使用小鼠END-2细胞条件培养基，可以显著增强分化过程。适当的暂时添加生长因子也是心脏分化的关键。Wnt信号通路是hPSC心脏分化的关键调节因子之一。在不同分化阶段调节Wnt信号活性足以驱动hPSC分化为心肌细胞。
ScienceCell的人多功能干细胞心肌细胞分化试剂盒（PSCCDK）专门设计用于高效地将hPSC分化为心肌细胞，将生长因子诱导与小分子对典型Wnt信号的时间调制相结合。该试剂盒在无血清和无喂养者的条件下，可在15天内将单层培养的多能干细胞有效转化为收缩心肌细胞。PSCCDK不需要中间胚状体的形成。该试剂盒包含3种分化培养基、1种基本培养基和1种生长补充剂。一个试剂盒足以区分2个12孔板的细胞。

PSCCDK试剂盒是使用在STEMium®培养基（Cat.#5801）中培养的hESC WA09（H9）开发的。我们一直从H9细胞中获得一片心肌细胞。使用该试剂盒，我们还能够从hiPSC株中获得收缩簇，该株是使用mRNA重编程技术从人类皮肤成纤维细胞中生成的。hPSC衍生的心肌细胞可以通过我们的心肌细胞选择性培养基（Cat.#5911）传代并进一步富集。

Human pluripotent stem cells (hPSC), including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC), possess the capability of differentiating into all derivatives of the three germ layers. Given that adult cardiomyocytes do not typically proliferate, hPSC can potentially provide an unlimited supply of cardiomyocytes. The cardiomyocyte derived from hPSC can be used for drug screening, toxicity testing, and developmental studies.

The spontaneous cardiac differentiation of hPSC is inefficient, with less than 1% of cardiomyocytes present in the culture. The differentiation process can be significantly enhanced by adding growth factors (BMP4, activin A, and FGF2 etc.), and small molecules, or using mouse END-2 cell-conditioned medium. The appropriate temporal addition of growth factors is also critic for cardiac differentiation. The Wnt signaling pathway is one of the key regulators in cardiac differentiation of hPSC. Modulating the Wnt signaling activity at different stages of differentiation is sufficient to drive hPSC to differentiate into cardiomyocytes.

ScienCell’s Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit (PSCCDK) is specially designed to differentiate hPSC to cardiomyocytes at a high efficiency, combining growth factor induction with the temporal modulation of canonical Wnt signaling by small molecules. The kit efficiently converts monolayer-cultured pluripotent stem cells to contracting cardiomyocytes within 15 days under serum-free and feeder-free conditions. With the PSCCDK there is no need for the intermediate embryoid body formation. The kit contains 3 differentiation media, 1 basal medium, and 1 growth supplement. One kit is enough to differentiate 2 12-well plates of cells.

The PSCCDK kit is developed using hESC WA09 (H9) cultured in STEMium® medium (Cat. #5801). We consistently get a sheet of cardiomyocytes from H9 cells. Using the kit, we are also able to derive contracting clusters from a hiPSC line which is generated from human dermal fibroblasts using mRNA reprogramming technology. The hPSC-derived cardiomyocyte cells can be passaged and further enriched using our Cardiomyocyte Selective Medium (Cat. #5911).

以下是ScienCell品牌部分干细胞相关产品，如需其他产品请联系本店客服。
ScienCell 0343  ScienCell 超低吸附细胞培养板（48孔）ULBCP-48 Ultra-Low Binding Culture Plate (48-well)
 ScienCell 0353 超低吸附细胞培养板（96孔）ULBCP-96, Ultra-Low Binding Culture Plate (96-well)
ScienCell 0373 ScienCell 超低结合培养板ULBC Plates Ultra-Low Binding Culture Plate
ScienCell 0383 超低结合培养板ULBC Plates Ultra-Low Binding Culture Plate 24孔/板
ScienCell 0423   0.2%明胶溶液（无菌）GSN，0.2% Gelatin Solution , sterile 100ml
 ScienCell 104-02 重组人 成纤维细胞生长因子-basic rhbFGF，Recombinant Human Fibroblast Growth Factor-basic, 10 μg
ScienCell 105-04 重组人表皮生长因子 rhEGF，Recombinant Human Epidermal Growth Factor 100 ug
ScienCell 108-02 重组人骨形成蛋白-2rhBMP-2，Recombinant Human Bone Morphogenetic Protein-2  2 ug
ScienCell 108-04 重组人骨形成蛋白-4 rhBMP-4, Recombinant Human Bone Morphogenetic Protein-4,  2 μg
 ScienCell 108-07 重组人骨形成蛋百-7 rhBNP-7，Recombinant Human Bone Morphogenetic Protein-7 2 ug
ScienCell 108-09 重组人NOGGIN蛋白rhNOGGIN，Recombinant Human NOGGIN  5 μg
ScienCell 5801 无血清，无滋养层人多能干细胞培养基STEMium 500ml
 ScienCell 5803 人胚胎干细胞解离液StmDS, StemDS? Human Embryonic Stem Cell Dissociation Solution  100 ml
ScienCell 5805 HDF衍生的人类诱导多能干细胞总RNA HIPSC-HDF Trna，HDF-derived Human Induced Pluripotent Stem Cell Total RNA 10μg
 ScienCell 5807 来自人类诱导多能干细胞（HIPSC）miRNA HIPSC-HDF miRNA，HDF-derived Human Induced Pluripotent Stem Cell (HIPSC) MicroRNA  1 μg
 ScienCell 5811 无异源人多能干细胞培养基STEMium-XF，STEMium?Human Pluripotent Stem Cell Growth Medium-Xeno 5811
 ScienCell 5821 人多能干细胞培养基-无动物成分，Human Pluripotent Stem Cell Growth Medium-animal component free 500ml
 ScienCell 5825 人胚胎干细胞H9总RNA HESC-H9tRNA，Human Embryonic Stem Cell H9 Total RNA 10μg
 ScienCell 5881 小鼠胚胎成纤维细胞-条件培养基MEF-cm，Mouse Embryonic Fibroblast-Conditioned Medium 100ml
 ScienCell 5891 碱性成纤维细胞生长添加物刺激 小鼠胚胎成纤维细胞条件培养基Begf-stdMEF-cm，bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium 100ml
 ScienCell 5901 人多能干细胞向心肌细胞分化试剂盒PSCCDK，Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit, 50 ml 50 ml
 ScienCell 5911 心肌细胞选择性培养基CSM，Cardiomyocyte Selective Medium 500ml
ScienCell 5931 多能干细胞诱导神经干细胞培养基PSCNIM，HPSC Neural Induction Medium 500ml