Human pluripotent stem cells (hPSC), including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC), possess the capability of differentiating into all derivatives of the three germ layers. Given that adult cardiomyocytes do not typically proliferate, hPSC can potentially provide an unlimited supply of cardiomyocytes. The cardiomyocyte derived from hPSC can be used for drug screening, toxicity testing, and developmental studies.
The spontaneous cardiac differentiation of hPSC is inefficient, with less than 1% of cardiomyocytes present in the culture. The differentiation process can be significantly enhanced by adding growth factors (BMP4, activin A, and FGF2 etc.), and small molecules, or using mouse END-2 cell-conditioned medium. The appropriate temporal addition of growth factors is also critic for cardiac differentiation. The Wnt signaling pathway is one of the key regulators in cardiac differentiation of hPSC. Modulating the Wnt signaling activity at different stages of differentiation is sufficient to drive hPSC to differentiate into cardiomyocytes.
ScienCell’s Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit (PSCCDK) is specially designed to differentiate hPSC to cardiomyocytes at a high efficiency, combining growth factor induction with the temporal modulation of canonical Wnt signaling by small molecules. The kit efficiently converts monolayer-cultured pluripotent stem cells to contracting cardiomyocytes within 15 days under serum-free and feeder-free conditions. With the PSCCDK there is no need for the intermediate embryoid body formation. The kit contains 3 differentiation media, 1 basal medium, and 1 growth supplement. One kit is enough to differentiate 2 12-well plates of cells.
The PSCCDK kit is developed using hESC WA09 (H9) cultured in STEMium® medium (Cat. #5801). We consistently get a sheet of cardiomyocytes from H9 cells. Using the kit, we are also able to derive contracting clusters from a hiPSC line which is generated from human dermal fibroblasts using mRNA reprogramming technology. The hPSC-derived cardiomyocyte cells can be passaged and further enriched using our Cardiomyocyte Selective Medium (Cat. #5911).
以下是ScienCell品牌部分干细胞相关产品,如需其他产品请联系本店客服。 ScienCell0343 ScienCell 超低吸附细胞培养板(48孔)ULBCP-48 Ultra-Low Binding Culture Plate (48-well) ScienCell0353超低吸附细胞培养板(96孔)ULBCP-96, Ultra-Low Binding Culture Plate (96-well) ScienCell0373ScienCell 超低结合培养板ULBC Plates Ultra-Low Binding Culture Plate ScienCell0383 超低结合培养板ULBC Plates Ultra-Low Binding Culture Plate24孔/板 ScienCell0423 0.2%明胶溶液(无菌)GSN,0.2% Gelatin Solution , sterile100ml ScienCell 104-02重组人 成纤维细胞生长因子-basic rhbFGF,Recombinant Human Fibroblast Growth Factor-basic, 10 μg ScienCell 105-04重组人表皮生长因子 rhEGF,Recombinant Human Epidermal Growth Factor100 ug ScienCell108-02重组人骨形成蛋白-2rhBMP-2,Recombinant Human Bone Morphogenetic Protein-2 2 ug ScienCell108-04 重组人骨形成蛋白-4 rhBMP-4, Recombinant Human Bone Morphogenetic Protein-4, 2 μg ScienCell108-07重组人骨形成蛋百-7 rhBNP-7,Recombinant Human Bone Morphogenetic Protein-72 ug ScienCell 108-09重组人NOGGIN蛋白rhNOGGIN,Recombinant Human NOGGIN 5 μg ScienCell5801无血清,无滋养层人多能干细胞培养基STEMium500ml ScienCell5803人胚胎干细胞解离液StmDS, StemDS? Human Embryonic Stem Cell Dissociation Solution 100 ml ScienCell5805 HDF衍生的人类诱导多能干细胞总RNA HIPSC-HDF Trna,HDF-derived Human Induced Pluripotent Stem Cell Total RNA10μg ScienCell5807来自人类诱导多能干细胞(HIPSC)miRNA HIPSC-HDF miRNA,HDF-derived Human Induced Pluripotent Stem Cell (HIPSC) MicroRNA 1 μg ScienCell5811无异源人多能干细胞培养基STEMium-XF,STEMium?Human Pluripotent Stem Cell Growth Medium-Xeno5811 ScienCell5821人多能干细胞培养基-无动物成分,Human Pluripotent Stem Cell Growth Medium-animal component free500ml ScienCell5825人胚胎干细胞H9总RNA HESC-H9tRNA,Human Embryonic Stem Cell H9 Total RNA10μg ScienCell5881小鼠胚胎成纤维细胞-条件培养基MEF-cm,Mouse Embryonic Fibroblast-Conditioned Medium100ml ScienCell5891 碱性成纤维细胞生长添加物刺激 小鼠胚胎成纤维细胞条件培养基Begf-stdMEF-cm,bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium100ml ScienCell5901人多能干细胞向心肌细胞分化试剂盒PSCCDK,Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit, 50 ml50 ml ScienCell5911心肌细胞选择性培养基CSM,Cardiomyocyte Selective Medium500ml ScienCell 5931多能干细胞诱导神经干细胞培养基PSCNIM,HPSC Neural Induction Medium500ml