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- 详细信息
- 文献和实验
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- 保存条件:
-20°C, sealed storage, away from moisture and light
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
959-81-9
- 规格:
200 mg
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4-Di-1-ASP
CAS No. : 959-81-9
MCE 国际站:4-Di-1-ASP
产品活性:4-Di-1-ASP 是一种苯乙烯基染料,可用作活脑组织中的神经胶质瘤细胞染色,用于细胞结构、活力、增殖及内吞、胞吐、吞噬作用分析,也可用于观察活细胞线粒体结构。4-Di-1-ASP 在显微镜成像时可发出绿色荧光 (λex /λem = 475/606 nm)。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1.Dissolve the dye in water and configure a mother liquor at a concentration of 2 mM.
2.Place the prepared cell sections in an incubator at 35°C, add the prepared dye solution to a final concentration of 1 μM and incubate with the stain for 10 min.
3.The prepared stained cell sections are placed on a microscope slide, cover with a coverslip and observe using a fluorescent confocal microscope with a maximum excitation wavelength of 475 nm and a maximum emission wavelength of 606 nm.
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文献和实验=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Forward: Score: 94.000000 Q:2 to 23 R:1363 to 1384 Align Len (22) (77.27%) (81.82%) Query: 3' AGUUGUAGUCAGA-CUAUUCGAU 5' |:||| || || ||||||||| Ref: 5' GCGACAGGAG-CUGGAUGAGUUG 3' Energy: -20.200001 kCal/Mol
Design of the Invader Genotyping Assay
, 47 bases PRIMER_PRODUCT_SIZE_RANGE=81-600 PRIMER_FIRST_BASE_INDEX=1 PRIMER_GC_CLAMP=1 PRIMER_OPT_SIZE=20 PRIMER_MAX_SIZE=25 PRIMER_MIN_SIZE=17 PRIMER_OPT_TM=60 PRIMER_MAX_TM=64 PRIMER_MIN_TM=58 PRIMER_MAX_DIFF=100 PRIMER_MIN_GC=30 PRIMER
电镜 纳米颗粒跟踪分析法:简称NTA,该方法能保证外泌体原始状态、检测速度快,检测后能提供外泌体粒径和浓度信息。 图四 NTA检测图 Western blot分子标志物检测:外泌体标志蛋白包括四跨膜蛋白家族,如CD9、CD63和CD81;细胞质蛋白,如肌动蛋白(Actin)和钙磷脂结合蛋白(Annexins);参与生物功能的分子,如凋亡转接基因2互作蛋白X(ALIX)、肿瘤易感基因101蛋白(TSG101)、热休克蛋白(HSP70、HSP90),以及细胞分泌的特异性蛋白。 图五
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