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Please store the product under the recommended conditions in the Certificate of Analysis.
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货期:14天
- 供应商:
MedChemExpress LLC
- CAS号:
2755154-93-7
- 规格:
电询
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Cyanine3 maleimide tetrafluoroborate
CAS No. : 2755154-93-7
MCE 国际站:Cyanine3 maleimide tetrafluoroborate
产品活性:Cyanine3 maleimide tetrafluoroborate 是一种巯基特异性标记试剂,λex 为 550 nm,λem 为 580 nm。Cyanine3 maleimide tetrafluoroborate 可用于蛋白质和肽标记。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of Protein:1.The protein concentration was adjusted to 1 mg/mL for 100 μL.2. A 100 μL aliquot of the protein solution was mixed with Cyanine3 maleimide tetrafluoroborate (8 μg) in DMSO (2 μL).3. Incubated overnight at 4 °C in the dark.4. Labeled proteins were desalted using a G25 desalting column to remove excessive Cyanine3 maleimide tetrafluoroborate.5. Fluorescence intensities (FI) of labeled proteins were measured at excitation and emission wavelengths of 550 nm and 570 nm, respectively.MCE has not independently confirmed the accuracy of these methods. They are for reference only.
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文献和实验., Alexa Fluor 488 carboxylic acid, succinimidyl ester [Invitrogen]; FITC [Thermo Fisher Scientific]; rhodamine‐maleimide [Biotium]) DMSO, anhydrous grade
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