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Please store the product under the recommended conditions in the Certificate of Analysis.
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MedChemExpress LLC
- CAS号:
216571-99-2
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询盘
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BODIPY FL Thapsigargin
CAS No. : 216571-99-2
MCE 国际站:BODIPY FL Thapsigargin
产品活性:BODIPY FL Thapsigargin 是一种有效的绿色荧光染料。BODIPY FL Thapsigargin 抑制肌浆/内质网中存在的细胞内 serca 型 Ca2+ 泵。BODIPY FL Thapsigargin 用于研究 Thapsigargin 在活细胞中的结合位点。
研究领域:Membrane Transporter/Ion Channel | Neuronal Signaling
作用靶点:Calcium Channel
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
BODIPY FL Thapsigargin:
1. Purified parasites are fixed with 1% formaldehyde in PBS (pH 8) for 1 h at room temperature and allows to adhere to poly-L-lysine-coated glass slides for 20 min Parasites are then permeated with 0.1% Triton X-100 for 5 min and blocked with 3% bovine serum albumin in PBS for 1 h.
2. Parasites were incubated at room temperature with a 1:100 dilution of the primary polyclonal antibody H-300 for 1 h, followed by a 1:100 dilution of Texa Red-coupled goat anti-rabbit IgG secondary antibody, and with 0.25 μM BODIPY FL TG for 5 min.
3. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
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文献和实验Immunofluorescence Microscopy of tissue culture cells
Immunofluorescence Microscopy of tissue culture cells These methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and indirect immunofluorescence staining of microtubules with anti-tubulin antibodies
fluorescent dye BODIPY FL l -cystine to label PEI and pLL. Our probe is activated under reductive conditions leading to strongly increased fluorescence intensity. Subsequently, we show how the intracellular route of polyplexes made from these labeled polymers
、与配体耦联、与肽耦联、与人工合成的寡聚核苷酸耦联的探针,可用于免疫组化、荧光原位杂交、受体标记等 2)FITC (fluorescent isothiocyanate) BODIPY FL 503/512 3)异硫氰基荧光素 494/518 4)TRITC 四甲基异硫氰基罗丹明 544/572 BODIPY TMR 543/569 5)Texas Red 德州红 595/615 BODIPY TR592/618 6)PE 藻红蛋白 565
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